In vivo experimental study of noninvasive insulin microinjection through hollow Si microneedle array

Resnik, Drago (author), Možek, Matej (author), Pečar, Borut (author), Janež, Andrej (author), Urbančič-Rovan, Vilma (author), Iliescu, Ciprian (author), Vrtačnik, Danilo (author)

An experimental study of in vivo insulin delivery through microinjection by using hollow silicon microneedle array is presented. A case study was carried out on a healthy human subject in vivo to determine the influence of delivery parameters on drug transfer efficiency. As a microinjection device, a hollow microneedle array (13 X 13 mm$^2$) having 100 microneedles (220 µm high, 130 µm-outer diameter and 50 µm-inner diameter) was designed and fabricated using classical microfabrication techniques. The efficiency of the delivery process was first characterized using methylene blue and a saline solution. Based on these results, the transfer efficiency was found to be predominantly limited by the inability of viable epidermis to absorb and allow higher drug transport toward the capillary-rich region. Two types of fast-acting insulin were used to provide evidence of efficient delivery by hollow MNA to a human subject. By performing blood analyses, infusion of more-concentrated insulin (200 IU/mL, international units (IU)) exhibited similar blood glucose level drop (5-7%) compared to insulin of standard concentration (100 IU/mL), however, significant increase of serum insulin (40-50%) with respect to the preinfusion values was determined. This was additionally confirmed by a distinctive increase of insulin to C-peptide ratio as compared to preinfusion ratio. Moreover, we noticed that this route of administration mimics a multiple dose regimen, able to get a "steady state" for insulin plasma concentration.

• Language: English
• Keywords: hollow Si microneedle array, microinjection, in vivo insulin delivery, drug transfer efficiency
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FE - Faculty of Electrical Engineering
• Publication status: Published
• Publication version: Version of Record
• Year: 2018
• Number of pages: 17 str.
• Numbering: Vol. 9, iss. 1, art. 40
• PID: 20.500.12556/RUL-130995
• UDC: 681.5:621.38
• ISSN on article: 2072-666X
• DOI: 10.3390/mi9010040
• COBISS.SI-ID: 33603801
• Publication date in RUL: 21.09.2021

Record is a part of a journal

• Title: Micromachines
• Shortened title: Micromachines
• Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
• ISSN: 2072-666X
• COBISS.SI-ID: 523276825

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 20.01.2018

Secondary language

• Language: Slovenian
• Keywords: votla Si mikronaična matrika, mikroinjektiranje, in vivo dajanje insulina, polje votlih Si mikrokonic, mikroinjiciranje, doziranje inzulina in-vivo, izkoristek vnosa

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: L2-4122
• Name: Mikrosistem za uvajanje zdravila

• Funder: ARRS - Slovenian Research Agency
• Project number: P2-0244
• Name: Mikrostrukture in nanostrukture