In vivo experimental study of noninvasive insulin microinjection through hollow Si microneedle array

Resnik, Drago (avtor), Možek, Matej (avtor), Pečar, Borut (avtor), Janež, Andrej (avtor), Urbančič-Rovan, Vilma (avtor), Iliescu, Ciprian (avtor), Vrtačnik, Danilo (avtor)

An experimental study of in vivo insulin delivery through microinjection by using hollow silicon microneedle array is presented. A case study was carried out on a healthy human subject in vivo to determine the influence of delivery parameters on drug transfer efficiency. As a microinjection device, a hollow microneedle array (13 X 13 mm$^2$) having 100 microneedles (220 µm high, 130 µm-outer diameter and 50 µm-inner diameter) was designed and fabricated using classical microfabrication techniques. The efficiency of the delivery process was first characterized using methylene blue and a saline solution. Based on these results, the transfer efficiency was found to be predominantly limited by the inability of viable epidermis to absorb and allow higher drug transport toward the capillary-rich region. Two types of fast-acting insulin were used to provide evidence of efficient delivery by hollow MNA to a human subject. By performing blood analyses, infusion of more-concentrated insulin (200 IU/mL, international units (IU)) exhibited similar blood glucose level drop (5-7%) compared to insulin of standard concentration (100 IU/mL), however, significant increase of serum insulin (40-50%) with respect to the preinfusion values was determined. This was additionally confirmed by a distinctive increase of insulin to C-peptide ratio as compared to preinfusion ratio. Moreover, we noticed that this route of administration mimics a multiple dose regimen, able to get a "steady state" for insulin plasma concentration.

• Jezik: Angleški jezik
• Ključne besede: hollow Si microneedle array, microinjection, in vivo insulin delivery, drug transfer efficiency
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2018
• Št. strani: 17 str.
• Številčenje: Vol. 9, iss. 1, art. 40
• PID: 20.500.12556/RUL-130995
• UDK: 681.5:621.38
• ISSN pri članku: 2072-666X
• DOI: 10.3390/mi9010040
• COBISS.SI-ID: 33603801
• Datum objave v RUL: 21.09.2021

Gradivo je del revije

• Naslov: Micromachines
• Skrajšan naslov: Micromachines
• Založnik: Multidisciplinary Digital Publishing Institute (MDPI)
• ISSN: 2072-666X
• COBISS.SI-ID: 523276825

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 20.01.2018

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: votla Si mikronaična matrika, mikroinjektiranje, in vivo dajanje insulina, polje votlih Si mikrokonic, mikroinjiciranje, doziranje inzulina in-vivo, izkoristek vnosa

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: L2-4122
• Naslov: Mikrosistem za uvajanje zdravila

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P2-0244
• Naslov: Mikrostrukture in nanostrukture