Liquid chromatography is one of the most powerful analytical methods in pharmaceutic industry. Identification of substances using chromatography requires retention time comparison of analyte and standard substance. Higher number of related substances, produced during synthesis of active pharmaceutical ingredients, must be analysed by liquid chromatography, therefore high number of standard substances are required which is not cost effective.
Some routine analyses do not require standard substance usage, which enables faster analytical procedures and better cost effectiveness, because purchasing of expensive standard substances is not necessary.
Objectives of this master thesis were development of chromatographic method for quantification of norfloxacine, enrofloxacine and ciprofloxacine, successful execution of forced degradation using different agents and determination of response factors and relative response factors of produced related substances on different concentration levels
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