Detekcija sproščenega heksanala s plinsko kromatografijo

Kuret, Tajda (avtor), Kolar, Mitja (mentor)

Heksanal (oz. heksanaladehid) je alkil aldehid, ki se uporablja v industriji arom za živila in produkte s sadnim okusom in vonjem. Je hlapna spojina s protimikrobnim delovanjem, zato se ga pogosto uporablja za zaviranje zorenja sadja in zelenjave. Namen diplomskega dela je določiti količino sproščenega heksanala s plinsko kromatografijo. Kot detektor smo uporabili masni spektrometer, ki ponuja visoko selektivnost in občutljivost. GC-MS pogoje smo optimirali tako, da smo dobili dovolj kvalitetne spektre posameznih spojin in s tem primerno ločitev komponent. Analizno metodo smo potrdili in pokazali njeno linearnost, natančnost in ponovljivost. Analizirali smo tudi tri realne vzorce encimov, ki sproščajo heksanal. Največ heksanala se je sprostilo pri mešanici encimov lipaze in lipoksidaze, v vzorcu same lipoksidaze pa nismo zasledili večjih količin sproščenega heksanala, tudi po daljšem časovnem obdobju.

• Jezik: Slovenski jezik
• Ključne besede: heksanal, GC, izbira topila, headspace analiza
• Vrsta gradiva: Diplomsko delo/naloga
• Tipologija: 2.11 - Diplomsko delo
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Leto izida: 2021
• PID: 20.500.12556/RUL-129641
• COBISS.SI-ID: 76826115
• Datum objave v RUL: 06.09.2021

KURET, Tajda, 2021, Detekcija sproščenega heksanala s plinsko  kromatografijo [na spletu]. Diplomsko delo. [Dostopano 28 marec 2025]. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=129641

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Detection of released hexanal with gas chromatography

Izvleček

Hexanal (or hexanaldehyde) is an alkylaldehyde used in the flavor industry for foods and products with a fruity taste and aroma. It is a naturally volatile compound with antimicrobial activity, which is why it is often used to inhibit the ripening of fruits and vegetables. The aim of this work is to determine the amount of hexanal released by gas chromatography. Te detector used is a mass spectrometer, which offers high selectivity and sensitivity. GC-MS conditions were optimized to achieve sufficient quality of the spectra of the individual compounds and thus adequate separation of the components. The analytical method was validated to demonstrate its linearity, accuracy and repeatability. We also analyzed three real samples of hexanal releasing enzymes. Most of the hexanal was released by the mixture of lipase and lipoxidase enzymes, and no large amounts of released hexanal were detected in the lipoxidase sampel itself, even after a long time.

• Ključne besede: hexanal, GC, solvent, headspace analysis