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Targeted lipid analysis of haemolytic mycelial extracts of Aspergillus niger
ID Novak, Maruša (Author), ID Sepčić, Kristina (Author), ID Kraševec, Nada (Author), ID Križaj, Igor (Author), ID Maček, Peter (Author), ID Anderluh, Gregor (Author), ID Guella, Graziano (Author), ID Mancini, Ines (Author)

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Abstract
Ethanolic extracts of mycelia from Aspergillus niger (strain N402) grown in liquid media were observed to have haemolytic activity on bovine erythrocytes. This haemolytic activity decreased significantly during the time of growth (1–3 days). Moreover, when A. niger was grown on carbon-deprived medium, the efficiency of this haemolytic activity in the ethanolic extracts was much lower than when grown in carbon-enriched medium, and became almost undetectable after 3 days of growth in carbon-deprived medium. The lipid composition of these ethanolic extracts was analysed by liquid chromatography–electrospray ionisation tandem mass spectrometry. This haemolytic activity can be mainly linked to the relative levels of the molar ratios of the unsaturated fatty acids and lysophosphatidylcholines.

Language:English
Keywords:Aspergillus niger, haemolytic activity, fatty acids, lipid composition, liquid chromatography-selectrospray ionisation tandem mass spectrometry
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
FKKT - Faculty of Chemistry and Chemical Technology
Publication status:Published
Publication version:Version of Record
Year:2014
Number of pages:Str. 9051-9069
Numbering:Vol. 19, iss. 7
PID:20.500.12556/RUL-129221 This link opens in a new window
UDC:577.1
ISSN on article:1420-3049
DOI:10.3390/molecules19079051 This link opens in a new window
COBISS.SI-ID:3158095 This link opens in a new window
Publication date in RUL:30.08.2021
Views:747
Downloads:132
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Record is a part of a journal

Title:Molecules
Shortened title:Molecules
Publisher:MDPI
ISSN:1420-3049
COBISS.SI-ID:18462981 This link opens in a new window

Licences

License:CC BY 3.0, Creative Commons Attribution 3.0 Unported
Link:https://creativecommons.org/licenses/by/3.0/deed.en
Description:You are free to reproduce and redistribute the material in any medium or format. You are free to remix, transform, and build upon the material for any purpose, even commercially. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Licensing start date:01.07.2014

Projects

Funder:ARRS - Slovenian Research Agency
Project number:J1-4305
Name:Spremembe lipidnih membran pri bolezni

Funder:ARRS - Slovenian Research Agency
Project number:P1-0207
Name:Toksini in biomembrane

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