Targeted lipid analysis of haemolytic mycelial extracts of Aspergillus niger

Novak, Maruša (author), Sepčić, Kristina (author), Kraševec, Nada (author), Križaj, Igor (author), Maček, Peter (author), Anderluh, Gregor (author), Guella, Graziano (author), Mancini, Ines (author)

Ethanolic extracts of mycelia from Aspergillus niger (strain N402) grown in liquid media were observed to have haemolytic activity on bovine erythrocytes. This haemolytic activity decreased significantly during the time of growth (1–3 days). Moreover, when A. niger was grown on carbon-deprived medium, the efficiency of this haemolytic activity in the ethanolic extracts was much lower than when grown in carbon-enriched medium, and became almost undetectable after 3 days of growth in carbon-deprived medium. The lipid composition of these ethanolic extracts was analysed by liquid chromatography–electrospray ionisation tandem mass spectrometry. This haemolytic activity can be mainly linked to the relative levels of the molar ratios of the unsaturated fatty acids and lysophosphatidylcholines.

• Language: English
• Keywords: Aspergillus niger, haemolytic activity, fatty acids, lipid composition, liquid chromatography-selectrospray ionisation tandem mass spectrometry
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty; FKKT - Faculty of Chemistry and Chemical Technology
• Publication status: Published
• Publication version: Version of Record
• Year: 2014
• Number of pages: Str. 9051-9069
• Numbering: Vol. 19, iss. 7
• PID: 20.500.12556/RUL-129221
• UDC: 577.1
• ISSN on article: 1420-3049
• DOI: 10.3390/molecules19079051
• COBISS.SI-ID: 3158095
• Publication date in RUL: 30.08.2021

Record is a part of a journal

• Title: Molecules
• Shortened title: Molecules
• Publisher: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licences

• License: CC BY 3.0, Creative Commons Attribution 3.0 Unported
• Link: https://creativecommons.org/licenses/by/3.0/deed.en
• Description: You are free to reproduce and redistribute the material in any medium or format. You are free to remix, transform, and build upon the material for any purpose, even commercially. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
• Licensing start date: 01.07.2014

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: J1-4305
• Name: Spremembe lipidnih membran pri bolezni

• Funder: ARRS - Slovenian Research Agency
• Project number: P1-0207
• Name: Toksini in biomembrane