Determination of refractive index, size, and solid content of monodisperse polystyrene microsphere suspensions for the characterization of optical phantoms

Naglič, Peter (avtor), Zelinskyi, Yevhen (avtor), Likar, Boštjan (avtor), Bürmen, Miran (avtor)

Monodisperse polystyrene microspheres are often utilized in optical phantoms since optical properties such as the scattering coefficient and the scattering phase function can be calculated using the Mie theory. However, the calculated values depend on the inherent physical parameters of the microspheres which include the size, refractive index, and solid content. These parameters are often provided only approximately or can be affected by long shelf times. We propose a simple method to obtain the values of these parameters by measuring the collimated transmission of polystyrene microsphere suspensions from which the wavelength-dependent scattering coefficient can be calculated using the Beer-Lambert law. Since a wavelength-dependent scattering coefficient of a single suspension is insufficient to uniquely derive the size, refractive index and solid content by the Mie theory, the crucial and novel step involves suspending the polystyrene microspheres in aqueous sucrose solutions with different sucrose concentrations that modulates the refractive index of the medium and yields several wavelength-dependent scattering coefficients. With the proposed method, we are able to obtain the refractive index within 0.2% in the wavelength range from 500 to 800 nm, the microsphere size to approximately 15 nm and solid content within 2% of their respective reference values.

• Jezik: Angleški jezik
• Ključne besede: forward scattering, Mie theory, multiple scattering, refractive index, scattering measurement
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: Str. 1901-1918
• Številčenje: Vol. 11, no. 4, art. 387619
• PID: 20.500.12556/RUL-128815
• UDK: 535
• ISSN pri članku: 2156-7085
• DOI: 10.1364/BOE.387619
• COBISS.SI-ID: 12953940

Avtorske pravice

V članku navedeno: "© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement"; s povezavo https://www.osapublishing.org/library/license_v1.cfm. (3. 8. 2021)

• Datum objave v RUL: 03.08.2021

NAGLIČ, Peter, ZELINSKYI, Yevhen, LIKAR, Boštjan in BÜRMEN, Miran, 2020, Determination of refractive index, size, and solid content of monodisperse polystyrene microsphere suspensions for the characterization of optical phantoms. Biomedical optics express [na spletu]. 2020. Vol. 11, no. 4,  387619, p. 1901–1918. [Dostopano 29 maj 2025]. DOI 10.1364/BOE.387619. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=128815

Gradivo je del revije

• Naslov: Biomedical optics express
• Skrajšan naslov: Biomed. opt. express
• Založnik: Optica
• ISSN: 2156-7085
• COBISS.SI-ID: 24857383

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: usmerjeno sipanje, teorija Mie, večkratno sipanje, lomni količnik, meritve sipanja