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Helical organization of microtubules occurs in a minority of tunneling membrane nanotubes in normal and cancer urothelial cells
Resnik, Nataša (Author), Prezelj, Tim (Author), Luca, Giulia M. R. de (Author), Manders, Erik (Author), Polishchuk, Roman S. (Author), Veranič, Peter (Author), Erdani-Kreft, Mateja (Author)

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Abstract
Tunneling membrane nanotubes (TnTs) are membrane protrusions connecting nearby or distant cells in vitro and in vivo. Functions of TnTs in cellular processes are various and rely on TnT structure, which also depends on cytoskeletal composition. In the present study, we focused on the organization of microtubules (MTs) and intermediate filaments (IFs) in TnTs of urothelial cells. We analysed TnTs of normal porcine urothelial cells, which morphologically and physiologically closely resemble normal human urothelial cells, and of cancer cells derived from invasive human urothelial neoplasm. Wide-field fluorescence, confocal and super-resolution microscopy techniques, together with image analyses and 3D reconstructions enlightened specific MT-IF organization in TnTs, and for the first time revealed that MTs and IFs co-occur in the majority of normal and cancer urothelial cell TnTs. Our findings show that in the initiation segment of TnTs, MTs are cross-linked with each other into filamentous network, however in the middle and the attaching segment of TnT, MTs can helically enwrap IFs, the phenomenon that has not been shown before within the TnTs. In this study, we assess MT-IF co-occurrence in TnTs and present evidence that such helical organization of MTs enwrapping IFs is only occurring in a minority of the TnTs. We also discuss the possible cell-biological and physiological reasons for helical organization of MTs in TnTs.

Language:English
Keywords:microtubules, tunneling membrane nanotubes, cancer urothelial cells, helical organization, normal human urothelial cells, initiation segment, super-resolution microscopy techniques
Work type:Article (dk_c)
Tipology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Year:2018
Number of pages:8 str.
Numbering:Vol. 8, art. 17133
UDC:616.6
ISSN on article:2045-2322
DOI:10.1038/s41598-018-35370-y This link opens in a new window
COBISS.SI-ID:34052057 This link opens in a new window
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Downloads:42
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Record is a part of a journal

Title:Scientific reports
Shortened title:Sci. rep.
Publisher:Nature Publishing Group
ISSN:2045-2322
COBISS.SI-ID:18727432 This link opens in a new window

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:J3-7494
Name:Tunelske membranske nanocevke za inovativno zdravljenje raka sečnega mehurja

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P3-0108
Name:Diferenciacija urotelijskih celic

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:20.11.2018

Secondary language

Language:Slovenian
Keywords:mikrotubulari, tunelske membranske nanocevke, rakave urotelijske celice

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