20.500.12556/RUL-128742 Helical organization of microtubules occurs in a minority of tunneling membrane nanotubes in normal and cancer urothelial cells Tunneling membrane nanotubes (TnTs) are membrane protrusions connecting nearby or distant cells in vitro and in vivo. Functions of TnTs in cellular processes are various and rely on TnT structure, which also depends on cytoskeletal composition. In the present study, we focused on the organization of microtubules (MTs) and intermediate filaments (IFs) in TnTs of urothelial cells. We analysed TnTs of normal porcine urothelial cells, which morphologically and physiologically closely resemble normal human urothelial cells, and of cancer cells derived from invasive human urothelial neoplasm. Wide-field fluorescence, confocal and super-resolution microscopy techniques, together with image analyses and 3D reconstructions enlightened specific MT-IF organization in TnTs, and for the first time revealed that MTs and IFs co-occur in the majority of normal and cancer urothelial cell TnTs. Our findings show that in the initiation segment of TnTs, MTs are cross-linked with each other into filamentous network, however in the middle and the attaching segment of TnT, MTs can helically enwrap IFs, the phenomenon that has not been shown before within the TnTs. In this study, we assess MT-IF co-occurrence in TnTs and present evidence that such helical organization of MTs enwrapping IFs is only occurring in a minority of the TnTs. We also discuss the possible cell-biological and physiological reasons for helical organization of MTs in TnTs. microtubules tunneling membrane nanotubes cancer urothelial cells helical organization normal human urothelial cells initiation segment super-resolution microscopy techniques mikrotubulari tunelske membranske nanocevke rakave urotelijske celice true false true Angleški jezik Slovenski jezik Članek v reviji 2021-07-27 10:00:34 2021-07-27 10:00:38 2022-09-07 04:16:56 0000-00-00 00:00:00 2018 0 0 8 str. Vol. 8, art. 17133 Nov. 2018 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.6 2045-2322 10.1038/s41598-018-35370-y 34052057 RAZ_Resnik_Natasa_2018.pdf RAZ_Resnik_Natasa_2018.pdf 1 3840984E814829BF06146E953DE06057 5b02fda8fc82d2afb8dccff6c3b6f1d4b54869b9ca99ab1a602cf68c540e631a 907d767a-eeb0-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145584 https://www.nature.com/articles/s41598-018-35370-y 0 c674e3ea-eeb0-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145585 Medicinska fakulteta 0 0 0