Short microsecond pulses achieve homogeneous electroporation of elongated biological cells irrespective of their orientation in electric field

Dermol-Černe, Janja (avtor), Batista Napotnik, Tina (avtor), Reberšek, Matej (avtor), Miklavčič, Damijan (avtor)

In gene electrotransfer and cardiac ablation with irreversible electroporation, treated muscle cells are typically of elongated shape and their orientation may vary. Orientation of cells in electric field has been reported to affect electroporation, and hence electrodes placement and pulse parameters choice in treatments for achieving homogeneous effect in tissue is important. We investigated how cell orientation influences electroporation with respect to different pulse durations (ns to ms range), both experimentally and numerically. Experimentally detected electroporation (evaluated separately for cells parallel and perpendicular to electric field) via Ca$^2$$^+$ uptake in H9c2 and AC16 cardiomyocytes was numerically modeled using the asymptotic pore equation. Results showed that cell orientation affects electroporation extent: using short, nanosecond pulses, cells perpendicular to electric field are significantly more electroporated than parallel (up to 100-times more pores formed), and with long, millisecond pulses, cells parallel to electric field are more electroporated than perpendicular (up to 1000-times more pores formed). In the range of a few microseconds, cells of both orientations were electroporated to the same extent. Using pulses of a few microseconds lends itself as a new possible strategy in achieving homogeneous electroporation in tissue with elongated cells of different orientation (e.g. electroporation-based cardiac ablation).

• Jezik: Angleški jezik
• Ključne besede: electroporation, gene therapy, cardiac ablation
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: 17 str.
• Številčenje: Vol. 10, art. 9149
• PID: 20.500.12556/RUL-128666
• UDK: 602.621
• ISSN pri članku: 2045-2322
• DOI: 10.1038/s41598-020-65830-3
• COBISS.SI-ID: 18364163
• Datum objave v RUL: 22.07.2021

DERMOL-ČERNE, Janja, BATISTA NAPOTNIK, Tina, REBERŠEK, Matej in MIKLAVČIČ, Damijan, 2020, Short microsecond pulses achieve homogeneous electroporation of elongated biological cells irrespective of their orientation in electric field. Scientific reports [na spletu]. 2020. Vol. 10, no. 9149. [Dostopano 22 julij 2025]. DOI 10.1038/s41598-020-65830-3. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=128666

Gradivo je del revije

• Naslov: Scientific reports
• Skrajšan naslov: Sci. rep.
• Založnik: Nature Publishing Group
• ISSN: 2045-2322
• COBISS.SI-ID: 18727432

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 04.06.2020

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: elektroporacija, genska terapija, srčna ablacija