Two marker and two selection genes were transformed into pre-prepared tobacco leaf explants (Nicotiana tabacum L.) by Agrobacterium tumefaciens (A. t.) strain LBA4404, which included plasmid pART27 2mgfp5 ER or pCAMBIA1390 DsRed. Phenotypic expression of gfp or DsRed marker transgenes was studied in live tobacco cells that had synthesized green or red fluorescent protein. To separate the transformed cells and tissues from the non-transformed ones, we used the selection transgenes nptII and hptII resistance to the antibiotic kanamycin or hygromycin. After infection of the explants by A. t. and plasmid pART27 2mgfp5 ER, the regeneration efficiency was 82.65 % and after infection with the A. t. pCAMBIA1390 DsRed 77.89 %. Among the vital regenerants we considered those that had roots and upright leaves. There were 63 (77.78 %) on kanamycin medium. These were also indicators of the success of nptII transgene transformation. After transformation of the explants by A. t. pCAMBIA1390 DsRed was formed 51 viable regenerants. These regenerants were able to degrade the active ingredient hygromycin. The transformation success rate was 68.92 % for the hptII transgene. At 4 regenerants (10 %) fragments of 211 bp length characteristic of the DsRed transgene did not amplify, despite the presence of a red fluorescent protein.
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