In the Western world, cannabis gained more importance after 1851, when it was included in the American Pharmacopoeia. Recently, more and more food and cosmetic products containing cannabinoids are appearing on the market. In particular, there is talk of therapeutic and analgesic effects of certain cannabinoids, such as CBD, CBG, CBN. According to European guidelines, the content of psychoactive ∆^9-THC in the final products should not exceed 0,2 % in the dry matter of the plant. To ensure the safety and efficacy of cannabis preparations, it is necessary to properly characterize the products and quantify the active substances in them, in particular to determine the legally controversial ∆^9-THC.
In the master's thesis, we developed methods for determination of ∆^9-THC in traces by various chromatographic methods for quality and stability control of natural and synthetic cannabinoid products. Using HPLC-UV/VIS, a chromatographic method to determine traces of ∆^9-THC was developed and validated. In order to develop more sensitive method, we optimized the parameters of the mass spectrometer on the LC MS/MS system and developed and partially validated the method. The results of the determination of ∆^9 THC in CBD sample by the mentioned methods were comparable. The use of GC FID was also tested, but it was not suitable for determining such low ∆^9 THC levels.