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Lignin intermediates lead to phenyl acid formation and microbial community shifts in meso- and thermophilic batch reactors
Prem, Eva Maria (Avtor), Mutschlechner, Mira (Avtor), Stres, Blaž (Avtor), Illmer, Paul (Avtor), Wagner, Andreas Otto (Avtor)

URLURL - Izvorni URL, za dostop obiščite https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-020-01855-0 Povezava se odpre v novem oknu

Izvleček
Background Lignin intermediates resulting from lignocellulose degradation have been suspected to hinder anaerobic mineralisation of organic materials to biogas. Phenyl acids like phenylacetate (PAA) are early detectable intermediates during anaerobic digestion (AD) of aromatic compounds. Studying the phenyl acid formation dynamics and concomitant microbial community shifts can help to understand the microbial interdependencies during AD of aromatic compounds and may be beneficial to counteract disturbances. Results The length of the aliphatic side chain and chemical structure of the benzene side group(s) had an influence on the methanogenic system. PAA, phenylpropionate (PPA), and phenylbutyrate (PBA) accumulations showed that the respective lignin intermediate was degraded but that there were metabolic restrictions as the phenyl acids were not effectively processed. Metagenomic analyses confirmed that mesophilic genera like Fastidiosipila or Syntrophomonas and thermophilic genera like Lactobacillus, Bacillus, Geobacillus, and Tissierella are associated with phenyl acid formation. Acetoclastic methanogenesis was prevalent in mesophilic samples at low and medium overload conditions, whereas Methanoculleus spp. dominated at high overload conditions when methane production was restricted. In medium carbon load reactors under thermophilic conditions, syntrophic acetate oxidation (SAO)-induced hydrogenotrophic methanogenesis was the most important process despite the fact that acetoclastic methanogenesis would thermodynamically be more favourable. As acetoclastic methanogens were restricted at medium and high overload conditions, syntrophic acetate oxidising bacteria and their hydrogenotrophic partners could step in for acetate consumption. Conclusions PAA, PPA, and PBA were early indicators for upcoming process failures. Acetoclastic methanogens were one of the first microorganisms to be impaired by aromatic compounds, and shifts to syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis occurred in thermophilic reactors. Previously assumed associations of specific meso- and thermophilic genera with anaerobic phenyl acid formation could be confirmed.

Jezik:Angleški jezik
Ključne besede:Bio-methane, Phenyl acids, Anaerobic digestion, Lignin intermediates, Amplicon sequencing
Vrsta gradiva:Znanstveno delo (r2)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FGG - Fakulteta za gradbeništvo in geodezijo
Leto izida:2021
Št. strani:Str. 1-23
Številčenje:art. 27, ǂVol.ǂ14
UDK:591
ISSN pri članku:1754-6834
DOI:10.1186/s13068-020-01855-0 Povezava se odpre v novem oknu
COBISS.SI-ID:48398851 Povezava se odpre v novem oknu
Število ogledov:122
Število prenosov:25
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Gradivo je del revije

Naslov:Biotechnology for biofuels
Skrajšan naslov:Biotechnol. biofuels
Založnik:BioMed Central
ISSN:1754-6834
COBISS.SI-ID:515780121 Povezava se odpre v novem oknu

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:mikrobiologija, lignin, bioplin, fenilna kislina

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