Separation and purification of biomacromolecules based on microfluidics

Vicente, Filipa A. (author), Plazl, Igor (author), Ventura, Sónia P. M. (author), Žnidaršič Plazl, Polona (author)

Separation and purification of biomacromolecules either in biopharmaceuticals and fine chemicals manufacturing, or in diagnostics and biological characterization, can substantially benefit from application of microfluidic devices. Small volumes of equipment, very efficient mass and heat transfer together with high process control result in process intensification, high throughputs, low energy consumption and reduced waste production as compared to conventional processing. This review highlights microfluidics-based separation and purification of proteins and nucleic acids with the focus on liquid–liquid extractions, particularly with biocompatible aqueous two-phase systems, which represent a cost-effective and green alternative. A variety of microflow set-ups are shown to enable sustainable and efficient isolation of target biomolecules both for preparative, as well as for analytical purposes.

• Language: English
• Keywords: microflow systems, biomacromolecules, aqueous two-phase systems, downstream processing
• Work type: Article
• Typology: 1.02 - Review Article
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: Str. 4391-4410
• Numbering: Vol. 22, iss. 14
• PID: 20.500.12556/RUL-127087
• UDC: 66.02
• ISSN on article: 1463-9262
• DOI: 10.1039/c9gc04362d
• COBISS.SI-ID: 1538561987
• Publication date in RUL: 17.05.2021

Record is a part of a journal

• Title: Green chemistry
• Shortened title: Green chem.
• Publisher: Royal Society of Chemistry
• ISSN: 1463-9262
• COBISS.SI-ID: 24129285

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 19.03.2020

Secondary language

• Language: Slovenian
• Keywords: mikropretočni sistemi, biomakromolekule, dvofazni vodni sistemi, zaključni procesi