Separation and purification of biomacromolecules based on microfluidics
Vicente, Filipa A. (Author), Plazl, Igor (Author), Ventura, Sónia P. M. (Author), Žnidaršič Plazl, Polona (Author)

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Separation and purification of biomacromolecules either in biopharmaceuticals and fine chemicals manufacturing, or in diagnostics and biological characterization, can substantially benefit from application of microfluidic devices. Small volumes of equipment, very efficient mass and heat transfer together with high process control result in process intensification, high throughputs, low energy consumption and reduced waste production as compared to conventional processing. This review highlights microfluidics-based separation and purification of proteins and nucleic acids with the focus on liquid–liquid extractions, particularly with biocompatible aqueous two-phase systems, which represent a cost-effective and green alternative. A variety of microflow set-ups are shown to enable sustainable and efficient isolation of target biomolecules both for preparative, as well as for analytical purposes.

Keywords:microflow systems, biomacromolecules, aqueous two-phase systems, downstream processing
Work type:Article (dk_c)
Tipology:1.02 - Review Article
Organization:FKKT - Faculty of Chemistry and Chemical Technology
Number of pages:Str. 4391-4410
Numbering:Vol. 22, iss. 14
ISSN on article:1463-9262
DOI:10.1039/c9gc04362d This link opens in a new window
COBISS.SI-ID:1538561987 This link opens in a new window
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Record is a part of a journal

Title:Green chemistry
Shortened title:Green chem.
Publisher:Royal Society of Chemistry
COBISS.SI-ID:24129285 This link opens in a new window

Document is financed by a project

Funder:Drugi - Drug financer ali več financerjev
Funding Programme:CICECO-Aveiro Institute of Materials
Project no.:UIDB/50011/2020 & UIDB/50011/2020

Funder:FCT - Fundação para a Ciência e a Tecnologia, I.P.
Project no.:IF/00402/ 2015

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P2-0191
Name:Kemijsko inženirstvo

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:N2-0067
Name:Večstopenjska sinteza z MIO-encimi v kontinuirnem mikroreaktorskem sistemu

Funder:EC - European Commission
Funding Programme:H2020
Project no.:811040
Name:Chair Of Micro Process Engineering and TEchnology


License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:19.03.2020

Secondary language

Keywords:mikropretočni sistemi, biomakromolekule, dvofazni vodni sistemi, zaključni procesi

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