20.500.12556/RUL-127087 Separation and purification of biomacromolecules based on microfluidics Separation and purification of biomacromolecules either in biopharmaceuticals and fine chemicals manufacturing, or in diagnostics and biological characterization, can substantially benefit from application of microfluidic devices. Small volumes of equipment, very efficient mass and heat transfer together with high process control result in process intensification, high throughputs, low energy consumption and reduced waste production as compared to conventional processing. This review highlights microfluidics-based separation and purification of proteins and nucleic acids with the focus on liquid–liquid extractions, particularly with biocompatible aqueous two-phase systems, which represent a cost-effective and green alternative. A variety of microflow set-ups are shown to enable sustainable and efficient isolation of target biomolecules both for preparative, as well as for analytical purposes. microflow systems biomacromolecules aqueous two-phase systems downstream processing mikropretočni sistemi biomakromolekule dvofazni vodni sistemi zaključni procesi true false false Angleški jezik Slovenski jezik Članek v reviji 2021-05-17 09:55:12 2021-05-17 09:55:12 2022-09-06 03:49:42 0000-00-00 00:00:00 2020 0 0 Str. 4391-4410 iss. 14 Vol. 22 21 Jul. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.02 1463-9262 10.1039/c9gc04362d 1538561987 RAZ_Vicente_Filipa_A._2020.pdf RAZ_Vicente_Filipa_A._2020.pdf 1 88CCA7D13C43461D9102CDD6AE732607 791567315addc56ee56fb54c16896d62740d900d381f53fdbac88f13635d874a f69e9519-b6e6-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=143300 https://pubs.rsc.org/en/content/articlelanding/2020/gc/c9gc04362d#!divAbstract 1 29ee553c-b6e5-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=143299 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0