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Copolymeric hydrogel-based immobilization of yeast cells for continuous biotransformation of fumaric acid in a microreactor
ID
Menegatti, Tadej
(
Author
),
ID
Žnidaršič Plazl, Polona
(
Author
)
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MD5: 29243511D31BD25D08EFF805675E5458
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https://www.mdpi.com/2072-666X/10/12/867
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Abstract
Although enzymatic microbioreactors have recently gained lots of attention, reports on the use of whole cells as biocatalysts in microreactors have been rather modest. In this work, an efficient microreactor with permeabilized Saccharomyces cerevisiae cells was developed and used for continuous biotransformation of fumaric into industrially relevant L-malic acid. The immobilization of yeast cells was achieved by entrapment in a porous structure of various hydrogels. Copolymers based on different ratios of sodium alginate (SA) and polyvinyl alcohol (PVA) were used for hydrogel formation, while calcium chloride and boric or phenylboronic acid were tested as crosslinking agents for SA and PVA, respectively. The influence of hydrogel composition on physico-chemical properties of hydrogels prepared in the form of thin films was evaluated. Immobilization of permeabilized S. cerevisiae cells in the selected copolymeric hydrogel resulted in up to 72% retained fumarase activity. The continuous biotransformation process using two layers of hydrogels integrated into a two-plate microreactor revealed high space time yield of 2.86 g/(L·h) while no activity loss was recorded during 7 days of continuous operation.
Language:
English
Keywords:
microreactor
,
copolymeric hydrogel
,
immobilization
,
biotransformation
,
L-malic acid
,
whole-cell biocatalysis
,
Saccharomyces cerevisiae
,
microbioreactor
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
FKKT - Faculty of Chemistry and Chemical Technology
Publication status:
Published
Publication version:
Version of Record
Year:
2019
Number of pages:
12 str.
Numbering:
Vol. 10, iss. 12, art. 867
PID:
20.500.12556/RUL-127084
UDC:
66.098
ISSN on article:
2072-666X
DOI:
10.3390/mi10120867
COBISS.SI-ID:
1538481859
Publication date in RUL:
17.05.2021
Views:
1340
Downloads:
185
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Record is a part of a journal
Title:
Micromachines
Shortened title:
Micromachines
Publisher:
Multidisciplinary Digital Publishing Institute (MDPI)
ISSN:
2072-666X
COBISS.SI-ID:
523276825
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
10.12.2019
Secondary language
Language:
Slovenian
Keywords:
mikroreaktorji
,
kopolimerni hidrogeli
,
imobilizacija
,
biotransformacije
,
L-jabolčna kislina
,
celična biokataliza
,
Saccharomyces cerevisiae
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P2-0191
Name:
Kemijsko inženirstvo
Funder:
ARRS - Slovenian Research Agency
Project number:
N2-0067
Name:
Večstopenjska sinteza z MIO-encimi v kontinuirnem mikroreaktorskem sistemu
Funder:
ARRS - Slovenian Research Agency
Funding programme:
Young researchers
Funder:
EC - European Commission
Funding programme:
H2020
Project number:
811040
Name:
Chair Of Micro Process Engineering and TEchnology
Acronym:
COMPETE
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