Copolymeric hydrogel-based immobilization of yeast cells for continuous biotransformation of fumaric acid in a microreactor
Menegatti, Tadej (Author), Žnidaršič Plazl, Polona (Author)

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Although enzymatic microbioreactors have recently gained lots of attention, reports on the use of whole cells as biocatalysts in microreactors have been rather modest. In this work, an efficient microreactor with permeabilized Saccharomyces cerevisiae cells was developed and used for continuous biotransformation of fumaric into industrially relevant L-malic acid. The immobilization of yeast cells was achieved by entrapment in a porous structure of various hydrogels. Copolymers based on different ratios of sodium alginate (SA) and polyvinyl alcohol (PVA) were used for hydrogel formation, while calcium chloride and boric or phenylboronic acid were tested as crosslinking agents for SA and PVA, respectively. The influence of hydrogel composition on physico-chemical properties of hydrogels prepared in the form of thin films was evaluated. Immobilization of permeabilized S. cerevisiae cells in the selected copolymeric hydrogel resulted in up to 72% retained fumarase activity. The continuous biotransformation process using two layers of hydrogels integrated into a two-plate microreactor revealed high space time yield of 2.86 g/(L·h) while no activity loss was recorded during 7 days of continuous operation.

Keywords:microreactor, copolymeric hydrogel, immobilization, biotransformation, L-malic acid, whole-cell biocatalysis, Saccharomyces cerevisiae, microbioreactor
Work type:Article (dk_c)
Tipology:1.01 - Original Scientific Article
Organization:FKKT - Faculty of Chemistry and Chemical Technology
Number of pages:12 str.
Numbering:Vol. 10, iss. 12, art. 867
ISSN on article:2072-666X
DOI:10.3390/mi10120867 This link opens in a new window
COBISS.SI-ID:1538481859 This link opens in a new window
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Record is a part of a journal

Shortened title:Micromachines
COBISS.SI-ID:523276825 This link opens in a new window

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P2-0191
Name:Kemijsko inženirstvo

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:N2-0067
Name:Večstopenjska sinteza z MIO-encimi v kontinuirnem mikroreaktorskem sistemu

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Funding Programme:PhD Grant
Project no.:MR-51842

Funder:EC - European Commission
Funding Programme:H2020
Project no.:811040
Name:Chair Of Micro Process Engineering and TEchnology


License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:10.12.2019

Secondary language

Keywords:mikroreaktorji, kopolimerni hidrogeli, imobilizacija, biotransformacije, L-jabolčna kislina, celična biokataliza, Saccharomyces cerevisiae

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