20.500.12556/RUL-127084 Copolymeric hydrogel-based immobilization of yeast cells for continuous biotransformation of fumaric acid in a microreactor Although enzymatic microbioreactors have recently gained lots of attention, reports on the use of whole cells as biocatalysts in microreactors have been rather modest. In this work, an efficient microreactor with permeabilized Saccharomyces cerevisiae cells was developed and used for continuous biotransformation of fumaric into industrially relevant L-malic acid. The immobilization of yeast cells was achieved by entrapment in a porous structure of various hydrogels. Copolymers based on different ratios of sodium alginate (SA) and polyvinyl alcohol (PVA) were used for hydrogel formation, while calcium chloride and boric or phenylboronic acid were tested as crosslinking agents for SA and PVA, respectively. The influence of hydrogel composition on physico-chemical properties of hydrogels prepared in the form of thin films was evaluated. Immobilization of permeabilized S. cerevisiae cells in the selected copolymeric hydrogel resulted in up to 72% retained fumarase activity. The continuous biotransformation process using two layers of hydrogels integrated into a two-plate microreactor revealed high space time yield of 2.86 g/(L·h) while no activity loss was recorded during 7 days of continuous operation. microreactor copolymeric hydrogel immobilization biotransformation L-malic acid whole-cell biocatalysis Saccharomyces cerevisiae microbioreactor mikroreaktorji kopolimerni hidrogeli imobilizacija biotransformacije L-jabolčna kislina celična biokataliza Saccharomyces cerevisiae true false true Angleški jezik Slovenski jezik Članek v reviji 2021-05-17 09:01:25 2021-05-17 09:01:27 2023-03-20 08:44:04 0000-00-00 00:00:00 2019 0 0 12 str. iss. 12, art. 867 Vol. 10 Dec. 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.098 2072-666X 10.3390/mi10120867 1538481859 RAZ_Menegatti_Tadej_2019.pdf RAZ_Menegatti_Tadej_2019.pdf 1 29243511D31BD25D08EFF805675E5458 b6f5e343186671ee6d1af98bbf66969862c74ee5a9111afdf8a40d808b1ef439 cae9be8b-420e-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=150839 https://www.mdpi.com/2072-666X/10/12/867 1 a7b8057d-b6dd-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=143294 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0