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Poly-L-lysine as an effective and safe desquamation inducer of urinary bladder epithelium
Kerec Kos, Mojca (Author), Veranič, Peter (Author), Erman, Andreja (Author)

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Abstract
Induced desquamation of urinary bladder epithelial cells, also called urothelial cells, is frequently used in studies of bladder epithelial regeneration and also in treating recurrent bacterial cystitis. Positively charged polymer chitosan is known to cause large-scale desquamation of terminally differentiated urothelial cells called umbrella cells. Aiming to compare the desquamation ability of another polycation poly-L-lysine, we studied the effect of this polymer on the functional and structural integrity of the urothelium in ex vivo and in vivo experiments. The urothelium was analyzed by measuring transepithelial electrical resistance, and the structural changes of its luminal surface were analyzed with scanning electron microscopy. The results revealed a selective and concentration-dependent desquamation effect of poly-L-lysine on superficial urothelial cells followed by quick regeneration of the urothelium, which functionally and structurally recovers in 2 to 3 h after poly-L-lysine-induced injury. Poly-L-lysine was thus proven to be a promising polymer to be used when desquamation of urothelial cells is required in basic and potentially clinical studies.

Language:English
Keywords:poly-L-lysine, urothelium, transepithelial electrical resistance, cell desquamation, ex vivo, in vivo
Work type:Article (dk_c)
Tipology:1.01 - Original Scientific Article
Organization:FFA - Faculty of Pharmacy
MF - Faculty of Medicine
Year:2019
Number of pages:14 str.
Numbering:Vol. 11, iss. 9, art. 1506
UDC:615.362:612.461
ISSN on article:2073-4360
DOI:10.3390/polym11091506 This link opens in a new window
COBISS.SI-ID:4789617 This link opens in a new window
Views:180
Downloads:115
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Record is a part of a journal

Title:Polymers
Shortened title:Polymers
Publisher:Molecular Diversity Preservation International
ISSN:2073-4360
COBISS.SI-ID:517951257 This link opens in a new window

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P3-0108
Name:Diferenciacija urotelijskih celic

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P1-0189
Name:Farmacevtska tehnologija: od dostavnih sistemov učinkovin do terapijskih izidov zdravil pri otrocih in starostnikih

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:I0-0022
Name:Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL)

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:12.03.2021

Secondary language

Language:Slovenian
Keywords:urinoterapija, urotelij, in vivo

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