20.500.12556/RUL-125370 Poly-L-lysine as an effective and safe desquamation inducer of urinary bladder epithelium Induced desquamation of urinary bladder epithelial cells, also called urothelial cells, is frequently used in studies of bladder epithelial regeneration and also in treating recurrent bacterial cystitis. Positively charged polymer chitosan is known to cause large-scale desquamation of terminally differentiated urothelial cells called umbrella cells. Aiming to compare the desquamation ability of another polycation poly-L-lysine, we studied the effect of this polymer on the functional and structural integrity of the urothelium in ex vivo and in vivo experiments. The urothelium was analyzed by measuring transepithelial electrical resistance, and the structural changes of its luminal surface were analyzed with scanning electron microscopy. The results revealed a selective and concentration-dependent desquamation effect of poly-L-lysine on superficial urothelial cells followed by quick regeneration of the urothelium, which functionally and structurally recovers in 2 to 3 h after poly-L-lysine-induced injury. Poly-L-lysine was thus proven to be a promising polymer to be used when desquamation of urothelial cells is required in basic and potentially clinical studies. poly-L-lysine urothelium transepithelial electrical resistance cell desquamation ex vivo in vivo urinoterapija urotelij in vivo true false true Angleški jezik Slovenski jezik Članek v reviji 2021-03-12 12:11:04 2021-03-12 12:11:05 2022-09-05 03:50:26 0000-00-00 00:00:00 2019 0 0 14 str. iss. 9, art. 1506 Vol. 11 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.362:612.461 2073-4360 10.3390/polym11091506 4789617 517951257 RAZ_Kerec_Kos_Mojca_2019.pdf RAZ_Kerec_Kos_Mojca_2019.pdf 1 53647814A4CABBA95D1CFC9FB603F2D2 d09a7ad3ff86eaa9eab9061b355afb0f42269ffbbacde5e3810c18bdc6283f6c b2a284b4-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141069 https://www.mdpi.com/2073-4360/11/9/1506 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141068 Fakulteta za farmacijo Medicinska fakulteta 0 0 0