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Toksičnost nanodelcev za mišje mikroglia celice v razmerah in vitro
ID Babič, Lea (Author), ID Narat, Mojca (Mentor) More about this mentor... This link opens in a new window, ID Hafner Bratkovič, Iva (Comentor)

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Abstract
V sklopu te magistrske naloge smo raziskali vplive treh tipov nanodelcev na celično linijo mišjih možganskih makrofagov (mikroglijske celice) in na nevronsko celično linijo. Z dodajanjem CM-H2DCFDA barvila v medij smo spremljali sproščanje reaktivnih kisikovih spojin. Sproščanje citokinov smo pa smo detektirali z encimsko imunoadsorpcijsko metodo (ELISA). Po prekonočni inkubaciji celic z nanodelci smo s pregledom s presevnim elektronskim mikroskopom opazili internalizacijo nanodelcev za vse tipe nanodelcev pri izbranih koncentracijah. Ugotovili smo, da ob kratkotrajni izpostavljenosti celic testiranim nanodelcem pri izbranih koncentracijah le ti niso imeli direktnega vpliva na viabilnost celic pri nobeni celični liniji, kar smo preverjali z barvanjem celic s propidijevim jodidom, ki ob kratkotrajni inkubaciji vstopa le v mrtve celice. Prišli smo do zaključka, da ti trije tipi nanodelcev v danih koncentracijah ne vplivajo na viabilnost celic, hkrati pa tudi celice ne sproščajo povišanih količin citokinov in reaktivnih kisikovih spojin, ki bi lahko posredno poškodovale katere celice, opazna pa je internalizacija nanodelcev.

Language:Slovenian
Keywords:mikroglia celice, mišje mikroglia celice, nanodelci, preživetje, detekcija signalnih molekul, imunološke metode, ROS, ELISA
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Publisher:[L. Babič]
Year:2021
PID:20.500.12556/RUL-124543 This link opens in a new window
UDC:577.2:620.3:57.083
COBISS.SI-ID:50002435 This link opens in a new window
Publication date in RUL:31.01.2021
Views:1050
Downloads:120
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Secondary language

Language:English
Title:In vitro toxicity of nanoparticles in murine microglial cells
Abstract:
Despite the fact that the field of research of nanoparticle cytotoxicity is relatively wide, not much is known about the mechanisms of interactions between cells and nanoparticles. In the scope of this master's thesis we investigated interactions between nanoparticles and two cell lines, microglial cells and a neuronal cell line. We observed that the nanoparticles do not make an impact on the viability of both cell lines in short term incubation. We also followed the release of cytokines with enzyme-linked immunosorbent assay (ELISA) when microglial cells were incubated with nanoparticles and measured the reactive oxygen species formation, but we found that there were no significant increases in detection of both types of signalling molecules. Nevertheless, were able to prove that both cell lines are susceptible to internalization of nanoparticles as observed under the transmission electron microscope, which suggests that prolonged incubation with nanoparticles might lead to adverse effects.

Keywords:microglia cells, mice microglia cells, nanoparticles, viability, detection of signalling molecules, immunological methods, ROS, ELISA

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