Despite the fact that the field of research of nanoparticle cytotoxicity is relatively wide, not much is known about the mechanisms of interactions between cells and nanoparticles. In the scope of this master's thesis we investigated interactions between nanoparticles and two cell lines, microglial cells and a neuronal cell line. We observed that the nanoparticles do not make an impact on the viability of both cell lines in short term incubation. We also followed the release of cytokines with enzyme-linked immunosorbent assay (ELISA) when microglial cells were incubated with nanoparticles and measured the reactive oxygen species formation, but we found that there were no significant increases in detection of both types of signalling molecules. Nevertheless, were able to prove that both cell lines are susceptible to internalization of nanoparticles as observed under the transmission electron microscope, which suggests that prolonged incubation with nanoparticles might lead to adverse effects.
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