There are many different analytical procedures for carbohydrate determination, most of them used in the industry are chromatographic. In this diploma thesis I discuss chromatographic determination of carbohydrates with fluorescence detection. As part of this thesis I optimized the mobile phase gradient for the separation of a carbohydrate mixture, I compared three different labels used for carbohydrate detection with fluorescence and I checked the stability of the derivatives at room temperature and in a freezer. I also performed validation of the final method, which included checking of repeatability, linearity and determination of the limit of detection and quantification.
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