Glycans are carbohydrates that have an important structural and metabolic role in living organisms, which makes them commonly used in the pharmaceutical industry. As a part of my bachelor's thesis, I developed a method for the separation of glycans using mixed mode chromatography. The method was developed on maltodextrines, which were first labelled with fluorescence 2-AA reagent. I removed the excess of the fluorescence reagent using a solid phase extraction. The method was developed on the Thermo Fisher’s GlycanPac AXH-1 column, which has two mechanisms of separation, namely the ion-exchange and hydrophilic interactions. After its development, the method was validated with D-glucose and D-maltose. In order to validate the method, I checked its linearity, repeatability, sensitivity, accuracy and robustness.
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