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A molecular survey and phylogenetic analysis of porcine circovirus type 3 using oral fluid, faeces and serum
Plut, Jan (Author), Jamnikar Ciglenečki, Urška (Author), Golinar, Irena (Author), Knific, Tanja (Author), Štukelj, Marina (Author)

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Abstract
Background: Porcine circovirus type 3 is the most recently discovered porcine circovirus, and an emerging pathogen. In this study the status of its presence on some Slovenian farms is reported. The effectiveness of thevaccine against porcine circovirus type 2 was assessed against porcine circovirus type 3. Group samples of oral fluid, faeces and individual serum samples were taken from six different pig categories andtested for presence of viral DNA, using both real time and conventional PCR. Positive samples were subjected todirect Sanger sequencing. Nucleotide sequences were analyzed and compared to GenBank PCV3 sequences. Results: Positive samples were sent for genome sequencing, which confirmed the presence of virus in all differentpig categories on five farms. A high to moderate correlation of strong statistical significance was found betweenindividual serum samples, oral fluid and faeces. Slovenian PCV3 was found to be distributed in a way similar to thatof other countries. Slovenian PCV3 nt sequences are highly related, sharing more than 99.5% nt identity. On onefarm a commercially available vaccine against porcine circovirus type 2 was used on 3-week-old pigs. It did notaffect the presence of porcine circovirus type 3 in oral fluid or sera of any of the seven age groups of pigs, eachwith two control groups. Conclusions: The results constitute the first discovery of the virus in Slovenia. Genome sequencing has revealed ahigh degree of similarity between Slovenian and GenBank isolates.

Language:English
Keywords:viral disease, pig oral fluid, faeces, serum, PCV3, PCR, first report, Slovenia, Circoviridae infections - virology - veterinary, polymerse chain reaction - methods, phylogeny, genome viral - genetics, sequence analysis, DNA, swine
Work type:Article (dk_c)
Tipology:1.01 - Original Scientific Article
Organization:VF - Veterinary Faculty
Year:2020
Number of pages:9 str.
Numbering:Vol. 16, art. 281
UDC:636.4.09:616:57.08
ISSN on article:1746-6148
DOI:10.1186/s12917-020-02489-y This link opens in a new window
COBISS.SI-ID:24828675 This link opens in a new window
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Downloads:145
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Record is a part of a journal

Title:BMC veterinary research
Publisher:Springer Nature
ISSN:1746-6148
COBISS.SI-ID:515674393 This link opens in a new window

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P4-0092
Name:Zdravje živali, okolje in varna hrana

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:10.08.2020

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