20.500.12556/RUL-118032 A molecular survey and phylogenetic analysis of porcine circovirus type 3 using oral fluid, faeces and serum Background: Porcine circovirus type 3 is the most recently discovered porcine circovirus, and an emerging pathogen. In this study the status of its presence on some Slovenian farms is reported. The effectiveness of thevaccine against porcine circovirus type 2 was assessed against porcine circovirus type 3. Group samples of oral fluid, faeces and individual serum samples were taken from six different pig categories andtested for presence of viral DNA, using both real time and conventional PCR. Positive samples were subjected todirect Sanger sequencing. Nucleotide sequences were analyzed and compared to GenBank PCV3 sequences. Results: Positive samples were sent for genome sequencing, which confirmed the presence of virus in all differentpig categories on five farms. A high to moderate correlation of strong statistical significance was found betweenindividual serum samples, oral fluid and faeces. Slovenian PCV3 was found to be distributed in a way similar to thatof other countries. Slovenian PCV3 nt sequences are highly related, sharing more than 99.5% nt identity. On onefarm a commercially available vaccine against porcine circovirus type 2 was used on 3-week-old pigs. It did notaffect the presence of porcine circovirus type 3 in oral fluid or sera of any of the seven age groups of pigs, eachwith two control groups. Conclusions: The results constitute the first discovery of the virus in Slovenia. Genome sequencing has revealed ahigh degree of similarity between Slovenian and GenBank isolates. viral disease pig oral fluid faeces serum PCV3 PCR first report Slovenia Circoviridae infections - virology - veterinary polymerse chain reaction - methods phylogeny genome viral - genetics sequence analysis DNA swine true false true Angleški jezik Ni določen Članek v reviji 2020-08-14 13:59:32 2020-08-14 13:59:32 2022-08-30 04:01:08 0000-00-00 00:00:00 2020 0 0 9 str. art. 281 Vol. 16 2020 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 636.4.09:616:57.08 1746-6148 10.1186/s12917-020-02489-y 24828675 515674393 RAZ_Plut_Jan_2020.pdf RAZ_Plut_Jan_2020.pdf 1 6B0AA35DDA4A4DFB8BECFEACB6BCF03C 92d17ba4932dc58809d9bac6b9c82c1c1b4b9853048962bac0c0ced055dc7c1b b59210ca-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141146 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02489-y 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=131965 Veterinarska fakulteta 0 0 0