Vpliv molekulske korone nanomaterialov na viabilnost in proliferacijo celic in vitro

Tominc, Kaja (avtor), Drobne, Damjana (mentor)

Namen magistrske naloge je preveriti, kakšen vpliv ima biomolekularna korona izbranih nanomaterialov na vzajemno delovanje med nanomateriali in celicami. Testirali smo vpliv nanodelcev (ND) s predhodno ustvarjeno korono iz govejega serumskega albumina - BSA (predhodno oblečeni ND) ter tistih brez predhodno ustvarjene korone (predhodno neoblečeni ND) na človeških nevroblastomskih celicah SH-SY5Y. Testiranje smo izvajali s sedmimi različnimi nanodelci – tremi nanodelci TiO2 z različnimi lastnostmi ter nanodelci SiO2, Ag, CuO in ZnO. Za preverjanje vpliva korone smo opravili tri teste citotoksičnosti – test z barvilom resazurin, test privzema barvila nevtralno rdeče in test z barvilom Coomassie Brilliant Blue. Preverili smo tudi, koliko proteinov se je vezalo v korono posameznih delcev s testom BCA za določanje količine prisotnih proteinov. Predhodno oblečeni ZnO in CuO nanodelci so bili bolj citotoksični, kot tisti brez predhodno ustvarjene korone, ali pa je bila citotoksičnost obeh tretmajev podobna – pri nanodelcih Ag, ali pa citotoksičnega učinka nismo opazili - TiO2 ter SiO2. Vpliv prehodno ustvarjene korone na nanodelcih je bil bolj izrazit pri nanodelcih CuO in ZnO, od katerih se odtapljajo ioni. Predvidevamo, da so razlike v učinku predhodno oblečenih in neoblečenih ND ZnO in CuO, posledica olajšane endocitoze predhodno oblečenih ND, vendar tega nismo preverjali.

• Jezik: Slovenski jezik
• Ključne besede: nanodelci, biomolekularna korona, proteinska korona, citotoksičnost, SH-SY5Y, TiO2, CuO, ZnO, Ag, SiO2, resazurin, nevtralno rdeče, coomassie brilliant blue
• Vrsta gradiva: Magistrsko delo/naloga
• Organizacija: BF - Biotehniška fakulteta
• Leto izida: 2020
• PID: 20.500.12556/RUL-117229
• COBISS.SI-ID: 22350083
• Datum objave v RUL: 03.07.2020

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: The role of the molecular corona on nanomaterials in viability and proliferation of cells in vitro

Izvleček

The purpose of this master's thesis was to test the effect that the biomolecular corona of chosen nanomaterials has on the interaction between them and the cells. We tested two groups of nanoparticles. For the first group, we incubated pristine nanoparticles in bovine serum albumin - BSA before testing, so a corona formed. For the second group we used nanoparticles of the same material without a preformed corona. We tested the effect of each group of nanoparticles on human neuroblastoma cells SH-SY5Y and tested seven different types of nanoparticles – SiO2, Ag, CuO, ZnO, and three different TiO2 nanoparticle samples. To asses the effect of the preformed protein corona versus no preformed corona on SH-SY5Y cells we used several cytotoxicity tests – resazurin test, neutral red uptake test (NR) and coomassie brilliant blue test (CBB). We also evaluated the amount of proteins bound to nanoparticles with a preformed corona using a BCA Protein Assay Test. We found that the ZnO and CuO nanoparticles with a preformed corona had a greater cytotoxic effect on cells, than those without a preformed corona. The cytotoxic effect of nanoparticles with and without the corona was simmilar in the case of Ag nanoparticles, and for the TiO2 ter SiO2 we did not notice significant cytotoxic effects. The effect of a preformed corona was more noticable in CuO and ZnO nanoparticles, which dissolute into ions. We presume that the differences in the effect of nanoparticles with and without a preformed corona are due to increased endocytosis of nanoparticles with a preformed corona, but this was not tested.

• Ključne besede: nanoparticles, biomolecular corona, protein corona, cytotoxicity, SH-SY5Y, TiO2, CuO, ZnO, Ag, SiO2, resazurin, neutral red, coomassie brilliant blue