Genome sequence of an aichivirus detected in a common pipistrelle bat (Pipistrellus pipistrellus)

Diakoudi, Georgia (avtor), Jamnikar Ciglenečki, Urška (avtor), Lanave, Gianvito (avtor), Lelli, Davide (avtor), Martella, Vito (avtor), Kuhar, Urška (avtor)

The family Picornaviridae includes important human and animal pathogens that are associated with a wide range of diseases and, in some cases, have zoonotic potential. During epidemiological surveillance of bats, we identified, by next-generation sequencing (NGS) techniques, the presence of picornavirus RNA in a common pipistrelle bat (Pipistrellus pipistrellus). By coupling NGS, primer-walking strategies, and sequence-independent protocols to obtain the sequences of the 5′ and 3′ termini, we reconstructed the genome sequence of picornavirus strain ITA/2017/189/18-155. The genome of the bat picornavirus is 8.2 kb in length and encodes a polyprotein of 2462 amino acids. A comparison of polyprotein sequences revealed that this virus is distantly related (65.1% and 70.9% sequence identity at the nucleotide and amino acid level, respectively) to a bat aichivirus identified in 2010. Phylogenetic analysis showed that this picornavirus clustered closely with members of the genus Kobuvirus, which also includes human and animal aichiviruses. The identification of aichiviruses in several animal hosts is providing hints that will lead to an understanding of their origin and evolutionary patterns.

• Jezik: Angleški jezik
• Ključne besede: Genome sequence, picornavirus, bats
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2020
• Št. strani: Str. 1019-1022
• Številčenje: 165
• PID: 20.500.12556/RUL-116673
• UDK: 636.09:578
• ISSN pri članku: 0304-8608
• DOI: 10.1007/s00705-020-04548-z
• COBISS.SI-ID: 4935290
• Datum objave v RUL: 02.06.2020

Gradivo je del revije

• Naslov: Archives of virology
• Skrajšan naslov: Arch. virol.
• Založnik: Springer
• ISSN: 0304-8608
• COBISS.SI-ID: 25007872

Licence

• Licenca: CC BY-ND 4.0, Creative Commons Priznanje avtorstva-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nd/4.0/deed.sl
• Opis: Licenca Creative Commons Brez predelav dovoljuje uporabnikom ponovno distribucijo dela, vendar ne v spremenjeni obliki. Zahtevana je navedba avtorstva.
• Začetek licenciranja: 02.06.2020

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0092
• Naslov: Zdravje živali, okolje in varna hrana