Introduction: Breast cancer is the most common cancer in women and is therefore a major public health problem. Primary prevention, among which we count healthy lifestyle, prevents the occurence of cancer. Secondary prevention, which includes diagnostic tests, helps with the early diagnose of cancer. Breast self-examination is often recommended as part of secondary prevention. Purpose: The purpose of this study was to investigate lifestyle of women and their practice of breast self-examination. Methods: We conducted the cross-sectional, quantitative survey in February 2020. The research sample consisted of 234 women, who completed the questionnaire over social networks. Data was presented in the form of frequencies and percentages. Results: Tension, stress or high pressure is felt by 82% of respondents frequently and occasionally. About one fifth of respondents smoke. Alcohol has been drunk in the last year by 78% of respondents, 43% of whom drank more than the limits of low-risk drinking on a special occasion. Respondents have 3 to 4 meals a day, one of which is breakfast. They consume fruits and vegetables on a daily basis. Most respondents are physical active in various volumes. 32% of respondents do not perform breast self-examination. The most common reasons for not performing are forgetfulness and lack of knowledge. Women who perform breast self-examination do so without considering the day of the menstrual cycle. They perform self-examination when they remember. Most of the respondents palpate their breasts, while forgetting about palpating their armpits, viewing and comparing breasts in the mirror, and they do not consider the possibility of discharge. Discussion and conclusion: Cancer prevention is currently playing a key role in the fight against the disease. Respondents in our study should reduce the frequency of smoking, drinking alcohol on special occasions and increase the volume of physical activity. They can take responsibility for breast health through regular self-examination. Although most of the respondents examined their breasts, they didn't perform it properly. Nurses contribute significantly to women's health through health promotion and health education about lifestyle and breast self-examination.