Pollen is a natural bee product, used as a dietary supplement because of its positive properties. Pollen, collected by beekeepers from the bees, is called bee pollen. Bee bread is bee pollen, stored inside the comb, by the bee colony. The purpose of this research was to analyse the difference of collected bee pollen and bee bread in their nutritional and antioxidant values. Water, ash, protein and fat contents were analysed for collected bee pollen. The total carbohydrate content and nutritional value of bee pollen were calculated. We also analysed protein and fat content of bee bread. The antioxidant value was evaluated by analysing the content of total phenolic compounds and flavonoids, as well as FRAP and DPPH values. The data obtained for bee pollen and bee bread were compared, and also the influence of the hive and sampling time was evaluated. The results for bee bread were compared with respect to the depth of the honeycomb layer, hive and time of sampling. There were no statistically significant differences in protein and fat content between bee pollen and bee bread. Bee bread has higher antioxidant value compared to collected bee pollen. Our research showed no differences in protein and fat contents and antioxidant activity in bee bread relative to the hive or the depth of the layer of bee bread. The antioxidant value of bee bread depends on sampling time, which is probably related to the botanical origin of pollen.