Cabbage is one of the most important vegetables in the world. It is a biennial plant. Therefore the classical breeding procedure for new cabbage varieties is very time-consuming. Selfincompatibility is a characteristic of some flowering plants that could significantly shorten the breeding process. For this reason, the incompatibility class of 114 inbred lines of white cabbage from amplified DNA fragments using SRK-specific primers (S-locus receptor kinase) was determined. SRK gene is a female determinant and it is expressed in the pistil. Initially, we used 9 SRK-specific primers that amplify different domains of the SRK gene, and in the further experiment, 6 of them were eliminated because they did not function properly or were contaminated. We found out that 33.3% of the inbred lines belonged to class I. Using the sequence analysis, we analyzed the sequence of 66 inbred lines and classified them into subclasses. In class I, 4 different subclasses, and in class II, 2 subclasses were identified. At different locations across Slovenia, we planted a white cabbage plant together with a red cabbage plant for test-crossing. Seeds were obtained in only two locations. Seedlings were tested for self-pollination on the phenotypic level and with molecular markers. The percentage of self-pollination was very high in incompatibility class I, meaning that despite the strong selfincompatibility, a large number of plants were self-pollinated. Further research under more optimal conditions would be appropriate.
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