The development of information and communications technology in the field of road transport is fundamentally changing the entire transport system. Innovations in the field of road transport, i.e. Intelligent Transport Systems (ITS), offer many benefits for the environment, society, and the economy. For the penetration of ITS in Europe, Directive 2010/40/EU was adopted, which constitutes the legal framework for the deployment of ITS in road transport and for interfaces with other modes of transport. The latter was transposed in Slovenia in the amendment to the Roads Act on 26 June 2012. This master's thesis explores six research questions which are directly or indirectly related to the introduction of ITS in Slovenia and their interoperability in the EU through a qualitative methodology. The main objective of the transposition and implementation of Directive 2010/40/EU is to ensure the compatibility and interoperability of ITS and to ensure the continuity of ITS services throughout the EU. With the implementation of Directive 2010/40/EU, we are pursuing the objectives of an EU transport policy which aims to create a pan-European transport network with multimodal links and integrated ITS. Research findings on the transposition and implementation of Directive 2010/40/EU in Slovenia have shown that traffic bottlenecks will be eliminated, traffic congestion will be reduced, carbon dioxide emissions will be reduced, mobility will be improved, and integrated multimodal transport modes will be become available.