Vrednotenje vplivov antibiotikov na vodne bolhe in solinarske rake

Zalokar, Mojca (avtor), Kalčikova, Gabriela (mentor)

Uporaba antibiotikov je v zadnjem času zelo razširjena, zato tudi pogosto končajo v okolju. Ob kronični izpostavljenosti bakterije razvijejo odpornost na antibiotike in antibiotiki proti različnim bakterijam niso več učinkoviti. Tako je za zdravljenje različnih bolezni potrebno raziskati nove. Vpliv antibiotikov na različne organizme niso dobro poznani. Za testiranje strupenosti antibiotikov se lahko uporabljajo različni organizmi. Namen magistrske naloge je določanje akutnega vpliva antibiotikov na velike vodne bolhe in solinarske rakce, gojenje vodnih bolh in določevanje idealnih pogojev ter testiranje in primerjava občutljivosti. Testirani antibiotiki so bili tiamulin, levofloksacin, amoksicilin in klaritromicin. Gojenje in delo s solinarskimi rakci je bolj enostavno, njihova občutljivost pa je bila zelo nizka. Do koncentracije 250 mg/L ni bilo vpliva antibiotikov na solinarske rakce. Gojenje in delo z vodnimi bolhami zahteva veliko časa in spretnosti, organizmi pa so bistveno bolj občutljivi. Rezultati akutnih testov so pokazali, da so vodne bolhe občutljive predvsem na antibiotik tiamulin. Antibiotiki, ki jih najdemo v okolju so večinoma v koncentracijah do nekaj μg/L zato predvidevamo, da testirani antibiotiki nimajo neposrednega akutnega vpliva na te organizme.

• Jezik: Slovenski jezik
• Ključne besede: akutni testi, amoksicilin, antibiotik, klaritromicin, kronični testi, levofloksacin, solinarski rakci (Artemia salina), tiamulin, velika vodna bolha (Daphnia magna).
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Leto izida: 2019
• PID: 20.500.12556/RUL-111307
• COBISS.SI-ID: 1538440899
• Datum objave v RUL: 27.09.2019

ZALOKAR, Mojca, 2019, Vrednotenje vplivov antibiotikov na vodne bolhe in solinarske rake [na spletu]. Magistrsko delo. [Dostopano 11 april 2025]. Pridobljeno s: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=slv&id=111307

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Impact assessment of antibiotics on water fleas and brine shrimps

Izvleček

The use of antibiotics has recently become widespread, so they often occur in the environment. In the case of chronic exposure, bacteria can develop resistance to antibiotics, which means that antibiotics are no longer effective against various bacteria. It is therefore necessary to explore new antibiotics for the treatment of various diseases. The effects of antibiotics on different organisms are not well known. Various organisms may be used for testing of toxicity of antibiotics. The aim of this master's thesis is to assess the acute impact of antibiotics on water flea and brine shrimp, the cultivation of water flea and the determination of optimal conditions, and the testing and comparison of sensitivity. The tested antibiotics included tiamulin, levofloxacin, amoxicillin and clarithromycin. The cultivation and handling of brine shrimp is easier, but their sensitivity was very low. The antibiotics did not have any effect on brine shrimp up to a concentration of 250 mg/L. Cultivating and handling water fleas requires a lot of time and skills, and the organisms are significantly more sensitive. The results of acute tests have shown that water flea are primarily sensitive to the antibiotic tiamulin. Antibiotics found in the environment are mostly present in concentrations of up to a few μg/L; it is therefore assumed that the tested antibiotics do not have a direct acute effect on these organisms.

• Ključne besede: acute tests, amoxicillin, antibiotic, brine shrimp (Artemia salina), chronic tests, clarithromycin, levofloxacin, tiamulin, water flea (Daphnia magna).