With an aim to develop a compound with antitumor activity, we designed a synthetic pathway for the preparation of a diazenecarboxamide derivative with increased solubility and the ability to coordinate to transition metals. In the experimental part of diploma thesis, we prepared semicarbazide from phenyl isocyanate and 2-hydrazinopyridine and converted it into diazenecarboxamide by oxidation. From diazenecarboxamide and BOC-protected derivative of diazenecarboxamide, we attempted to prepare N-methylpyridinium salt with methyl iodide and dimethyl carbonate under various conditions. In this diploma thesis preparation, isolation, purification and analysis of semicarbazide and diazenecarboxamide is described. Attempts of preparation, isolation, purification and analysis of the products of methylation reactions are discussed. The products of the aforementioned reactions were analyzed with NMR and HRMS techniques. The product we wished to prepare was not obtained in any of the experiments.