Age-dependent expression of MyHC isoforms and lipid metabolism-related genes in the Longissimus dorsi muscle of wild and domestic pigs
Vrecl, Milka (Author), Cotman, Marko (Author), Uršič, Matjaž (Author), Čandek-Potokar, Marjeta (Author), Fazarinc, Gregor (Author)

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This study aimed to compare age-dependent changes in the relative expression of genes encoding myosin heavy chain (MyHC) isoforms and selected lipid metabolism-related genes in the longissimus dorsi muscle of wild pigs (WPs) and domestic pigs (DPs). Muscles sampled from postnatal day one as well as three-week-old and two-year-old animals were used in quantitative polymerase chain reaction (qPCR) assays, histological evaluations of succinate dehydrogenase (SDH) activity, and intra-myofiber lipid (IMFL) assessment. Expression of the MyHC isoforms displayed the most extensive age- and breed-dependent changes within the first three postnatal weeks. The MyHCembry level decreased significantly faster in the WPs than in the DPs. The relative MyHC-I and -IIa expression was significantly higher in the WPs, and MyHC-IIb was substantially higher in the DPs. The differences in MyHC expression corroborated the number of SDH-positive myofibers and IMFLs. Expression of the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α), peroxisome proliferator-activated receptor gamma (PPARγ) and lipoprotein lipase (LPL) genes displayed only age-related variations. In summary, the evidence is provided for accelerated postnatal myofiber transformation directed towards oxidative myofibers in WPs. The SDH activity/staining intensity largely reflected the expression of MyHCs, and not genes involved in lipid uptake and utilization.

Keywords:swine - growth & development, myofibers - genetics, polymerase chain reaction, histocytochemistry, myosin heavy chains, isoforms, lipid metabolism - genetics
Work type:Article (dk_c)
Tipology:1.01 - Original Scientific Article
Organization:VF - Veterinary Faculty
Number of pages:e10, str. 1-12
Numbering:Vol. 9, no. 1
ISSN on article:2076-2615
DOI:10.3390/ani9010010 Link is opened in a new window
COBISS.SI-ID:4739194 Link is opened in a new window
License:CC BY 4.0
This work is available under this license: Creative Commons Attribution 4.0 International
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Record is a part of a journal

Shortened title:Animals
Publisher:MDPI AG
COBISS.SI-ID:519120409 This link opens in a new window

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P4-0053
Name:Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:P4-0133
Name:Trajnostno kmetijstvo

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