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Multilokusna sekvenčna tipizacija fitoplazme 'Candidatus Phytoplasma solani' v slovenskih vinorodnih deželah
Mermal, Sara (Author), Dermastia, Marina (Mentor) More about this mentor... This link opens in a new window, Mehle, Nataša (Co-mentor)

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Abstract
'Candidatus Phytoplasma solani' je bakterija brez celične stene, ki je obligatni parazit rastlin. Je povzročiteljica bolezni na številnih rastlinah; na vinski trti na primer povzroča navadno trsno rumenico. S sekvenciranjem produktov ugnezdene verižne reakcije s polimerazo (nPCR) genov tuf, secY in stamp smo določili molekulsko raznolikost sevov 'Ca. P. solani' izoliranih iz vinske trte. Z analizo njihovega časovnega in geografskega pojavljanja v Sloveniji ter primerjavo rezultatov z rezultati podobnih analiz v Evropi, še posebej v okoliških državah, smo določili možno pot širjenja navadne trsne rumenice med Slovenijo in okoliškimi državami.

Language:Slovenian
Keywords:Candidatus Phytoplasma solani, fitoplazme, epidemiologija, PCR, secY, stamp, tuf
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2019
Publisher:[S. Mermal]
UDC:601.4:577.21:632.3(043.2)
COBISS.SI-ID:9243257 This link opens in a new window
Views:538
Downloads:179
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Secondary language

Language:English
Title:Multilocus sequence typing of phytoplasma ‘Candidatus Phytoplasma solani’ in slovenian wine regions
Abstract:
'Candidatus Phytoplasma solani' is a bacteria without a cell wall, which is an obligatory parasite of plants. It causes diseases in many plants, for example: Bois Noir on grapevine. By sequencing nested polymerase chain reaction products of tuf, secY and stamp genes, we determined the molecular diversity of 'Ca. P. solani' strains isolated from grapevines. By analyzing their occurrence in Slovenia on a geographical and time scale and then comparing the results with similar analysis in Europe, especially in surrounding countries, we determined possible pathways by which spreading of Bois Noir grapevine disease, between Slovenia and its neighbouring countries, can occur.

Keywords:Candidatus Phytoplasma solani, phytoplasma, epidemiology, PCR, secY, stamp, tuf

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