20.500.12556/RUL-108241 Multilokusna sekvenčna tipizacija fitoplazme 'Candidatus Phytoplasma solani' v slovenskih vinorodnih deželah Multilocus sequence typing of phytoplasma ‘Candidatus Phytoplasma solani’ in slovenian wine regions 'Candidatus Phytoplasma solani' je bakterija brez celične stene, ki je obligatni parazit rastlin. Je povzročiteljica bolezni na številnih rastlinah; na vinski trti na primer povzroča navadno trsno rumenico. S sekvenciranjem produktov ugnezdene verižne reakcije s polimerazo (nPCR) genov tuf, secY in stamp smo določili molekulsko raznolikost sevov 'Ca. P. solani' izoliranih iz vinske trte. Z analizo njihovega časovnega in geografskega pojavljanja v Sloveniji ter primerjavo rezultatov z rezultati podobnih analiz v Evropi, še posebej v okoliških državah, smo določili možno pot širjenja navadne trsne rumenice med Slovenijo in okoliškimi državami. 'Candidatus Phytoplasma solani' is a bacteria without a cell wall, which is an obligatory parasite of plants. It causes diseases in many plants, for example: Bois Noir on grapevine. By sequencing nested polymerase chain reaction products of tuf, secY and stamp genes, we determined the molecular diversity of 'Ca. P. solani' strains isolated from grapevines. By analyzing their occurrence in Slovenia on a geographical and time scale and then comparing the results with similar analysis in Europe, especially in surrounding countries, we determined possible pathways by which spreading of Bois Noir grapevine disease, between Slovenia and its neighbouring countries, can occur. Candidatus Phytoplasma solani fitoplazme epidemiologija PCR secY stamp tuf Candidatus Phytoplasma solani phytoplasma epidemiology PCR secY stamp tuf true false false [S. Mermal] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2019-06-22 07:45:04 2019-06-22 07:45:17 2022-08-20 03:45:17 0000-00-00 00:00:00 2019 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 601.4:577.21:632.3(043.2) 163884 9243257 7253.pdf 7253.pdf 1 BAEB5F0A4136FC208D8F02A5E13EB023 31c08e97611868c1f3848d4c35d025eef0c721c765a03522d80308923ae3ff15 9a249529-a1b6-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=119187 Biotehniška fakulteta 0 0 0