The aim of the master thesis was to determine the total phenolic content (TPC), antioxidant activity, 10-hydroxy-2-decenoic acid (10-HDA) content, and phenolic profile of royal jelly, and to investigate relationships among analysed parameters. Ten samples of Slovenian royal jelly and two samples of royal jelly of foreign origin were included in the study. The TPC in royal jelly samples was expressed in the equivalent of three standard compounds: gallic acid, hesperetin and hesperidin. The use of the Folin-Ciocalteu reagent in combination with UV-VIS spectrophotometry and solid phase extraction was found as an appropriate method for determination of TPC in royal jelly, as the results obtained were comparable with the results of foreign authors. The antioxidant activity of royal jelly was determined with two methods, such as DPPH∙ radical scavenging activity and ferric reducing antioxidant power (FRAP) assay. We confirmed the linear relationship among the methods used and proved that all samples of royal jelly were antioxidant effective. The 10-HDA content was in almost all samples above the prescribed limit of 1.4 g/100 g, only a sample of royal jelly of foreign origin did not meet the quality standard for the content of this fatty acid. By liquid chromatography-mass spectrometry, the content of individual phenolic compounds was determined and the results showed that phenolic acids and flavonoids were the most prevalent in the analysed samples of royal jelly. We have confirmed that the producer statistically significantly influence most of the parameters investigated.