Due to inadequate and uncritical use of antimicrobial drugs in animals, entering the human food chain, the number of resistant bacterial strains, isolated from humans as well as from the food, is increasing. Campylobacteriosis caused by Campylobacter jejuni is one of the most frequently reported gastrointestinal diseases in humans. The appearance of resistant bacterial strains increases the risk of ineffective treatment of the disease. Therefore, it is important to alert all stakeholders in the food production and supply chain, as well as doctors, to regularly carry out bacterial resistance tests on the most important antibiotics. In our experimental work, we tested the resistance of 8 strains of C. jejuni from the production (slaughterhouse) and 8 strains from the natural (surface water) environment against six antimicrobial drugs (erythromycin, ciprofloxacin, tetracycline, gentamicin, streptomycin nalidixic acids) on commercially prepared microtiter plates SensititreTM EUCAMP2. Both slaughtering (SS) and water (WS) strains have shown resistance to the same groups of antibiotics - ciprofloxacin (CIP), tetracycline (TET) and nalidixic acid (NAL). The proportion of resistant strains against individual AM was higher in slaughterhouse (CIP 87,5 %, TET 50%, NAL 50%) than water (CIP 25 %, TET 12,5 %, NAL 25 %) strains. Multidrug resistance was also present, which is in the case of slaughterhouse strains 87,5 % and 25 % in water strains, namely between CIP and TET (SS: 37,5 %) and CIP and NAL (SS: 50 %, WS: 25 %). We have come to the conclusion that the cause for the higher resistance levels of SS might be a higher exposure to antibiotics, and the cause of resistant WS is the transfer of resistant strains from the slaughterhouse to its surroundings.