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Metode za detekcijo sprememb DNA povzročenih s Crispr/Cas9.
Marolt, Nika (Author), Murovec, Jana (Mentor) More about this mentor... This link opens in a new window

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Abstract
Osrednja tema o kateri govorim v svoji diplomski nalogi so metode za detekcijo sprememb DNA povzročenih s CRISPR/Cas9. V uvodnem delu naloge opisujem razvoj odkritja naravnega CRISPR/Cas9 sistema skozi zgodovino. V nadaljnem delu sledi opis zgradbe CRISPR lokusa ter strukturne organizacijo in arhitekturo SpCas9, kjer sem se osredotočila na opis ključnih komponent za delovanje CRISPR/Cas9 sistema. V nadeljevanju sem predstavila način delovanja inženirskega CRISPR/Cas9 iz SpCas9 in njegovo uporabo kot molekularno orodje, s poudarkom na možnih spremembah v DNA in možne aplikacije. V drugi polovici diplomskega dela opisujem različne metode za detekcijo sprememb v DNA povzročenih s CRISPR/Cas9, možnosti njihove uporabe in prednosti ter pomankljivosti posameznih metod.

Language:Slovenian
Keywords:CRISPR, Cas9, metode detekciije, DNA spremembe, gensko inženirstvo, CRISPR-Cas9 struktura in mehanizem, CRISPR-Cas9 aplikacije
Work type:Bachelor thesis/paper (mb11)
Tipology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Year:2018
Publisher:[N. Marolt]
UDC:602.6(043.2)
COBISS.SI-ID:9008249 Link is opened in a new window
Views:557
Downloads:387
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Secondary language

Language:English
Title:Detection methods for DNA changes caused by CRISPR/Cas9
Abstract:
The main topic that I am discussing in my thesis is about methods for detecting changes in DNA caused by CRISPR/Cas9. In the introductory part of the paper I describe the development of the natural CRISPR/Cas9 system through history. The following is a description of the structure of the CRISPR locus and the structural organization and architecture of the SpCas9 domain, where I focused on describing the key components for the operation of the CRISPR/Cas9 system. Then I introduced how the engineered CRISPR/Cas9 from SpCas9 works and its use as a molecular tool, with an emphasis on possible changes in DNA and possible applications. In the second part of the diploma thesis I described various methods for detecting changes in DNA caused by CRISPR/Cas9, the possibilities of their use, and the advantages and disadvantages of individual methods.

Keywords:CRISPR, Cas9, detection methods, DNA changes, gene engineering, CRISPR-Cas9 structure and mechanism, CRISPR-Cas9 applications

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