20.500.12556/RUL-101949 Metode za detekcijo sprememb DNA povzročenih s Crispr/Cas9. Detection methods for DNA changes caused by CRISPR/Cas9 Osrednja tema o kateri govorim v svoji diplomski nalogi so metode za detekcijo sprememb DNA povzročenih s CRISPR/Cas9. V uvodnem delu naloge opisujem razvoj odkritja naravnega CRISPR/Cas9 sistema skozi zgodovino. V nadaljnem delu sledi opis zgradbe CRISPR lokusa ter strukturne organizacijo in arhitekturo SpCas9, kjer sem se osredotočila na opis ključnih komponent za delovanje CRISPR/Cas9 sistema. V nadeljevanju sem predstavila način delovanja inženirskega CRISPR/Cas9 iz SpCas9 in njegovo uporabo kot molekularno orodje, s poudarkom na možnih spremembah v DNA in možne aplikacije. V drugi polovici diplomskega dela opisujem različne metode za detekcijo sprememb v DNA povzročenih s CRISPR/Cas9, možnosti njihove uporabe in prednosti ter pomankljivosti posameznih metod. The main topic that I am discussing in my thesis is about methods for detecting changes in DNA caused by CRISPR/Cas9. In the introductory part of the paper I describe the development of the natural CRISPR/Cas9 system through history. The following is a description of the structure of the CRISPR locus and the structural organization and architecture of the SpCas9 domain, where I focused on describing the key components for the operation of the CRISPR/Cas9 system. Then I introduced how the engineered CRISPR/Cas9 from SpCas9 works and its use as a molecular tool, with an emphasis on possible changes in DNA and possible applications. In the second part of the diploma thesis I described various methods for detecting changes in DNA caused by CRISPR/Cas9, the possibilities of their use, and the advantages and disadvantages of individual methods. CRISPR Cas9 metode detekciije DNA spremembe gensko inženirstvo CRISPR-Cas9 struktura in mehanizem CRISPR-Cas9 aplikacije CRISPR Cas9 detection methods DNA changes gene engineering CRISPR-Cas9 structure and mechanism CRISPR-Cas9 applications true false false [N. Marolt] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2018-07-14 07:47:44 2018-07-14 07:47:48 2022-08-15 03:39:30 0000-00-00 00:00:00 2018 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 602.6(043.2) 151564 9008249 3442.pdf 3442.pdf 1 927F9254E82F20BFCB3C26A4ABEAA05A eacbd413448f1a49dbdaa1900f5062a039c879ab8610c5f6de3bcdfec80795af 7701159a-a1b5-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=112233 Biotehniška fakulteta 0 0 0