During ripening, many morphological and biochemical changes occur in the tomato fruit (Solanum lycopersicum L.). The research, which was conducted in 2015, consisted of monitoring morphometric and biochemical changes in the fruits of the hybrid varieties 'Tadim F1', 'Belle F1' and 'Amaneta F1', grafted onto the rootstock 'Beaufort'. During eight harvesting terms between July and October, we measured mass, width, height, firmness, dry matter content and the color of fruits. The content of total acids, sugars, phenols, nitrates and nitrites as well as the antioxidant potential was also determined. In all three varieties intensity of the red color is highest in the middle of the growing season. Intensity of the yellow color decreased during the growing season. The firmness of the fruits also decreased. Content of sugars, phenols, antioxidant potential and dry matter content increased during the growing season in all three varieties. Nitrates and nitrites content was low. In the varieties 'Amaneta F1' and 'Belle F1' total acids content was highest in the middle of the growing season. Variety 'Tadim F1' had highest total acids content in the beginnig of the growing season. Fruits of the variety 'Belle F1' had the highest phenols content and antioxidative potential, fruits of the variety 'Amaneta F1' highest sugar and dry matter content and lowest firmness. Fruits of the variety 'Tadim F1' had lowest mass and highest firmness. We concluded that the optimum harvesting period for all three varieties is the second half of August i.e. 165 to 180 days after planting.
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