In the study of fungicidal properties of hydrophilic extracts of Robinia pseudoacacia L. wood, the content of hydrophilic extracts, the content of total phenols and the content of robinetin (Rob) and dihydrorobinetin (DHR) were evaluated. The antioxidant potential of extracts was also studied. For obtaining hydrophilic extracts Soxhlet extraction with an acetone/water solvent was used. The extracts were further analysed with the UV-VIS spectrophotometer, where the content of the total phenols was determined. Detailed analysis of the robinia wood extractives was carried out on a system for high performance liquid chromatography (HPLC). Rob and DHR were qualitatively and quantitatively evaluated. The inhibitory effect of robinia extracts on the growth of wood-decay fungi was determined according to the mini blok test (modified protocol EN 113), which determines the effectiveness of wood protection in view of the loss of mass of wood samples due to the action of the fungus. The antioxidant activity of the robinia heartwood extracts was determined spectrophotometrically. Younger parts of the heartwood had higher content of hydrophilic extracts, total phenols, Rob and DHR, as compared to older parts. The content of hydrophilic extractives in the robinia wood is higher in the upper parts than the lower parts of the trunk. Sapwood and bark contain less hydrophilic extractives, total phenols, Rob and DHR than heartwood. Heartwood robinia extracts inhibit growth of the Gloeophyllum trabeum and Trametes versicolor. We have proven that apart from fungicidal effects, the extract of the robinia heartwood also has a high antioxidant potential.