Primerjava vpliva izbranih kovinskih in ogljikovih nanomaterialov na različne tipe človeških celic in vitro

Zrimšek, Mojca (author), Drobne, Damjana (mentor)

Testirali smo vplive neoplaščenih in z govejim serumskim albuminom (BSA) oplaščenih nanomaterialov (NM) amorfnega ogljika (CB), grafenovega oksida (GO), titanovega dioksida (TiO2) in titanovega dioksida, prevlečenega s polivinilpirolidonom (TiO2 PVP) na celične kulture človeških celic. Površino vsakega NM ob vstopu v biološki medij prekrijejo proteini in tvorijo proteinsko korono. Proteinska korona zmanjša vezavo NM na membrane celic in posledično zmanjša privzem NM v celice. Vpliv NM smo testirali na celicah HUVEC in THP-1. Uporabljeni celični kulturi nista imeli prisotne mikoplazme. Učinki ogljikovih in kovinskih NM so se razlikovali. Ugotovili smo, da NM motijo izvedbo spektrofotometričnih in spektrofluorimetričnih testov. Pri testiranju CB in GO sta se test MTT in test privzema barvila z nevtral rdečim izkazala kot primerna za testiranje citotoksičnosti, za testiranje TiO2 in TiO2 PVP pa je primeren test MTT. Preostali testi so vplivali na končne rezultate. Vpliv enega tipa NM ni imel podobnega učinka na različne vrste celic v kulturi. Celice THP-1 so bile bolj odporne na delovanje NM v primerjavi s celicami HUVEC. BSA je vplival na rezultate s celicami HUVEC, pri celicah THP-1 vpliv različnih koncentracij BSA ni imel učinka. Predhodno oblikovana korona iz BSA se je izkazala pri ogljikovih NM za boljšo pri zmanjševanju učinkov na celice HUVEC. CB, GO, TiO2 in TiO2 PVP imajo vpliv na celice HUVEC. CB prav tako vpliva na celice THP-1. GO, TiO2 in TiO2 PVP nimajo vpliva na celice THP-1, saj niso pokazali citotoksičnih učinkov. Uporaba NM oplaščenih z BSA je še vedno zmanjšala viabilnosti celic.

• Language: Slovenian
• Keywords: nanomateriali/CB/GO/TiO2/TiO2 PVP/celični liniji/proteinska korona
• Work type: Master's thesis/paper
• Organization: BF - Biotechnical Faculty
• Year: 2017
• PID: 20.500.12556/RUL-98323
• COBISS.SI-ID: 4514383
• Publication date in RUL: 26.11.2017

ZRIMŠEK, Mojca, 2017, Primerjava vpliva izbranih kovinskih in ogljikovih nanomaterialov na različne tipe človeških celic in vitro [online]. Master’s thesis. [Accessed 27 June 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=98323

Secondary language

• Language: English
• Title: COMPARING THE IMPACT OF SELECTED METAL AND CARBON NANOMATERIALS TO DIFFERENT TYPES OF HUMAN CELLS IN VITRO

Abstract

We tested the effects of nanomaterials (NM) carbon black (CB), graphene oxide (GO), titanium dioxide (TiO2), and titanium dioxide coated with polyvinylpyrrolidone (PVP TiO2) on human cell lines. The NM tested were added either in their pure forms, or previously coated with bovine serum albumine (BSA). Upon entering the biological medium, the surface of each NM is covered with proteins that form protein corona. Protein corona reduces the binding of NM to cell membranes and consequently reduces the uptake of NM into cells. The effect of NM was tested on HUVEC and THP-1 cells. Mycoplasma was not present in cell cultures. Effects of carbon and metal NM differed. We found that NM interfere with spectrophotometric and spectrofluorimetric tests. MTT assay and uptake of neutral red dye assay proved to be suitable for testing CB and GO cytotoxicity, while MTT assay is suitable for testing the effects of TiO2 and TiO2 PVP. The remaining tests affected the final results. The impact of one type NM did not have a similar effect on different types of cells. THP-1 cells were more resistant to the effect of NM in comparison with the HUVEC cells. BSA had an effect on HUVEC cells, while different concentrations of BSA did not cause any changes to the THP-1 cells. Carbon NM with BSA corona reduced cytotoxic effects on HUVEC cells. CB, GO, TiO2 and TiO2 PVP had an effect on HUVEC cells. CB also had an effect on THP-1 cells. GO, TiO2 and TiO2 PVP didn’t have any effect on THP-1 cells, because they did not show any cytotoxic effects. Use of BSA-coated NM still decreased cell viability.

• Keywords: nanomaterials/CB/GO/TiO2/TiO2 PVP/cell lines/protein corona