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Uporaba umetnih transkripcijskih regulatorjev za uravnavanje prepisovanja genov v sesalskih celicah
ID Verbič, Anže (Author), ID Marinšek Logar, Romana (Mentor) More about this mentor... This link opens in a new window, ID Jerala, Roman (Comentor)

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MD5: CE4BACF556862A0C510968CFDE120C3B
PID: 20.500.12556/rul/ed3c9b02-80b4-418b-9d69-d8664b8b3a75

Abstract
Uporaba načrtovanih DNA-vezavnih proteinov za natančen nadzor transkripcije poljubnih genov predstavlja enega izmed ključnih izzivov sintezne biologije. Do danes razviti pristopi temeljijo na genski fuziji efektorskih domen na načrtovane DNA-vezavne domene oziroma na kompetitivni vezavi načrtovanih DNA-vezavnih domen z endogenimi transkripcijskimi faktorji. V tej nalogi smo preučevali vpliv vezave načrtovanih TALE (ang. transcription activator-like effector) proteinov v bližino transkripcijskih aktivatorjev na osnovi cinkovih prstov Zif268 in Gal4. Ugotovili smo, da lahko z vezavo TALE proteinov v njuno bližino vplivamo na moč transkripcijske aktivacije, efekt pa je odvisen od pozicije in razdalje med vezavnimi mesti. TALE proteine lahko načrtujemo za vezavo v bližino endogenih transkripcijskih faktorjev, s čimer bi lahko nadzorovali izražanje endogenih genov celice.

Language:Slovenian
Keywords:sintezna biologija, TAL efektorji, umetni transkripcijski faktorji, upravljanje prepisovanja genov, DNA vezavni proteini, cinkovi prsti
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Publisher:[A. Verbič]
Year:2017
PID:20.500.12556/RUL-98321 This link opens in a new window
UDC:601.4:577.21
COBISS.SI-ID:4857208 This link opens in a new window
Publication date in RUL:26.11.2017
Views:1940
Downloads:660
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Secondary language

Language:English
Title:Use of artificial transcription regulators for transcriptional regulation in mammalian cells
Abstract:
One of the main current areas of research in synthetic biology is the development of artificially designed DNA binding proteins for use in transcription regulation of endogenous genes. Currently used systems of artificial transcription regulation rely on mechanisms of binding site competition and on the use of effector domains for activation of repression of transcription. In this work, we examined the effects of proximal binding of TALE (transcription activator-like effector) proteins, to zinc-finger transcription factors Zif268 and Gal4. Binding of TALE proteins in the vicinity of Zif268 and Gal4 was found to have an effect on their transcriptional activation, dependent of the position and distance between binding sites. As TALE proteins can be designed to bind in the vicinity of endogenous transcription factors, this method could be employed as a novel approach to transcriptional regulation.

Keywords:synthetic biology, TAL effectors, artificial transcription factors, transcriptional regulation, DNA binding proteins, zinc fingers

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