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Izvrednotenje akcesij šentjanževke (Hypericum perforatum L.) glede na ploidnost in vsebnost hiperforina
Lešnik, Leonida (Author), Bohanec, Borut (Mentor) More about this mentor... This link opens in a new window, Šircelj, Helena (Co-mentor)

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Abstract
Šentjanževka je fakultativno apomiktična vrsta, pri kateri je znanih več nivojev ploidnosti. V naravi jo najdemo tako v diploidnem kot tetraploidnem in heksaploidnem stanju. Najpogostejša oblika je tetraploid. Pomen šentjanževke kot zdravilne rastline je vezan na vsebnost večih farmakogenih substanc, najpomembnejša sta hipericin in hiperforin. Slednjemu zlasti pripisujejo antidepresivno delovanje. Medtem ko hipericin povzroča tudi fototoksičnost, tega problema pri hiperforinu ni, zato se pri novejših študijah šentjanževke osredotočajo predvsem na delovanje in vsebnost hiperforina. Hiperforin se v šentjanževki kopiči v prosojnih žlezah, ki so sestavljene iz votline, sekretornih celic in mezofilu podobnih celic, ter so vidne s prostim očesom. Njegova vsebnost je med akcesijami in sortami zelo različna. V nalogi smo proučili ploidnost znotraj akcesij šentjanževke in povezavo med ploidnostjo, velikostjo prosojnih žlez in vsebnostjo hiperforina. Rezultati kažejo, da je velikost prosojnih žlez odvisna od ploidnosti rastline, medtem ko pri vsebnosti hiperforina v povezavi s ploidnostjo ni bilo statistično značilnih razlik. Med ploidnostjo in povprečno površino peg obstaja močna pozitivna korelacija (ρ = 0,75), medtem ko med ploidnostjo in vsebnostjo hiperforina obstaja šibka korelacija (ρ = 0,24). Površina žlez niha od 9387,7 µm² (heksaploidna rastlina populacije 6/8 BF LP 2006) in 1575,9 µm² (diploidna rastlina populacije 6/4 BF LP 2006). Največja izmerjena vsebnost hiperforina je 18,49 mg/g suhe mase (heksaploidna rastlina linije Stamm 710), najmanjša pa 1,30 mg/g suhe mase (diploidna rastlina populacije 6/4 BF LP 2006.

Language:Slovenian
Keywords:šentjanževka, Hypericum perforatum, akcesije, ploidnost, hiperforin, prosojne žleze, mikrosateliti
Work type:Master's thesis/paper (mb22)
Tipology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Year:2017
Publisher:[L. Lešnik]
UDC:582.681.95:633.88:631.526.32:575.822(043.2)
COBISS.SI-ID:8835961 This link opens in a new window
Views:1093
Downloads:361
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Secondary language

Language:English
Title:Evaluation of St. John's Worth (Hypericum perforatum L.) accessions in relation to ploidy and hyperforin content
Abstract:
St. John's Wort is a facultative apomictic species known to exist in different ploidy levels. In nature, diploid, tetraploid and hexaploid forms can be found. The most common form is tetraploid. Importance of St. John's Wort as medicinal plants is related to possesion of several pharmaceutical substances, the most important being hypericin and hyperforin. The latter is known as putative antidepressant. While in hypericin can cause phototoxicity, hyperforin not and therefore recent studies of St. John's are predominantly focused on studies of hyperforin content. Hyperforin in the St. John's wort accumulates in translucent glands, which are composed of cavities, the secretory cells and mesophyll-like cells, which are visible to the naked eye. The amount of hyperforin varies considerably among accessions. Therefore, we examined the ploidy of accessions and relations between ploidy, transparent gland size and content of hyperforin. The results show that transparent gland size depends on ploidy, but relation between content of hyperforin and ploidy didn’t give us statistically significant differences. There is a strong positive correlation between the ploidy and transparent gland size (ρ = 0.75), whereas there is a weak correlation between the ploidy and the hyperforin content (ρ = 0.24). The size of transparent gland varies from 9387.7 µm² (hexaploid plant 6/8 BF LP 2006) and 1575.9 µm² (diploid plant 6/4 BF LP 2006). The maximum measured hyperforin content is 18.49 mg/g dry mass (hexaploid plant Stamm 710) and the minimum is 1.30 mg/g dry weight (diploid plant 6/4 BF LP 2006).

Keywords:St. John's Wort, Hypericum perforatum, accessions, ploidy, hyperforin, translucent gland, microsatellites

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