Cadavers represent a uniqe and promising source of stem cell which are usefull for many different researchs as well as regenerative medicine. This is importaint especially when there is limited number of donors, small amount of material and difficulties associated with purification. In our research we investigated if we can obtain cells from post mortem mouse tissue 3, 6 and 9 days after death. We used tissue from hippocampus, olfactory bulb, muscle and adipose tissue. We also investigated the inpact of temperature of storage and the form of storage on cell acquisition. In these cells we further analyzed gene expression of 84 key genes for cellular differentiation with RT-qPCR. We found out, that storage temperature has significant impact on acquisition of cells, because we were able to obtain cells only where tissue was stored at 4°C and not on room temperature. It turned out, that tissue from olfactory bulb gave the most positive results with cell growth and the poorly growth was in adipose tissue. We weren't able to obtain cells from any tissue 9 days after death and our results show that form of tissue storage hasn't got any significant impact. Analysis of gene expression has shown very low expression. There were expressed both, genes for terminal differentiated cells as well for progenitor cells. Most commonly expressed genes were Nanog, Cd34, Sox 7, Eno1, Msln, Gad2, Ptcra, Gad1, Gfap, Alb, Sftpb, G6pc and Tat. Since there was no typical pattern of expression we can not confirm the pluripotency of the cells we had obtain.
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