Genotipizacija bakterije Chlamydia trachomatis s sekvenciranjem

Ferko, Marina (author), Keše, Darja (mentor), Turk, Martina (reviewer)

Chlamydia trachomatis je obvezna znotrajcelična bakterija, za katero je značilen edinstven dvofazni razvojni krog. Zaradi prilagoditve na znotrajcelični parazitizem je družina Chlamydiaceae izgubila številne gene, kar je vodilo v zmanjševanje velikosti njihovega genoma. Serovari imajo genom velik približno 1 Mb. Sevi so razvrščeni v serovare na osnovi nukleotidnih razlik zaporedja v genu ompA, ki kodira poglavitno beljakovino zunanje membrane. Bakterija parazitira samo pri človeku in povzroča očesne in genitalne okužbe. Delimo jo lahko v tri biovare, ki slonijo na serovarih, en je povezan s trahomom (serovari A−C), drug z urogenitalnimi infekcijami (serovari D−K) in tretji z dimeljskim limfogranulomom (serovari L1−L3). Chlamydia trachomatis je najpogostejši bakterijski povzročitelj spolno prenosljivih bolezni v svetu. V magistrski nalogi nas je zanimalo, kateri genotipi C. trachomatis najpogosteje povzročajo okužbe. V raziskavi smo uporabili 126 izolatov C. trachomatis, pridobljenih iz kužnin bolnikov v letu 2014. Izvedli smo genotipizacijo gena ompA bakterije C. trachomatis s sekvenciranjem. Rezultati so pokazali, da je bil najbolj razširjen genotip E (51,6 %), sledil mu je genotip F (20,2 %), G (11,3 %), K (8,1 %), D (4%), J (2,4 %), H (0,8 %) in Ia ter Ja (0,8 %). Rezultati genotipizacije naše raziskave so bili primerljivi z nedavnimi rezultati različnih študijskih skupin v Evropi.

• Language: Slovenian
• Keywords: klamidije, Chlamydia trachomatis, spolno prenosljive bakterijske okužbe, genotipizacija, sekvenciranje, gen ompA
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Place of publishing: Ljubljana
• Publisher: [M. Ferko]
• Year: 2017
• Number of pages: XI, 56 f., [15] f. pril.
• PID: 20.500.12556/RUL-96461
• UDC: 579.61:616.98:579.882:577.2.088
• COBISS.SI-ID: 4820344
• Publication date in RUL: 02.10.2017

Secondary language

• Language: English
• Title: Genotyping of bacteria Chlamydia trachomatis by sequencing

Abstract

Chlamydia trachomatis is a compulsory intracellular bacterium which is characterized by a unique biphasic developmental cycle. Chlamydiaceae have undergone a massive genome reduction during the patho-gen’s ancient transition to the intracellular habitat. Serovars have a genome which is the size of about 1 Mb. Strains are classified into serovars based on nucleotide sequence differences in ompA, the gene that encodes the major outer membrane protein. Bacterium exclusively infects humans and cause ocular and genital infections. Chlamydia trachomatis is divided into three distinct biovars, based on serovars; one is associated with trachoma (serovars A–C) another with urogenital infections (serovars D–K) and the third with lymphogranuloma venereum (serovars L1–L3). Chlamydia trachomatis is a leading cause of sexually transmitted bacterial disease worldwide. In our study we wanted to find out which genotypes of C. trachomatis most frequently cause infections in our population. We analysed 126 isolates of bacterium C. trachomatis obtained from the patients in the year 2014 by genotyping the gene ompA with sequencing. The results showed that the most frequent genotype was E (51.6 %), followed by F (20.2 %), G (11.3 %), K (8.1 %), D (4 %), J (2.4 %), H (0.8 %), Ia and Ja (0.8 %). The results of our study were comparable with those of other research groups in Europe in the recent years.

• Keywords: chlamydias, Chlamydia trachomatis, sexually transmitted bacterial diseases, genotyping, sequencing, gene ompA