Molekulska raznolikost populacij fitoplazme 'Candidatus Phytoplasma solani' v slovenskih vinorodnih deželah

Vidmar, Sara (author), Dermastia, Marina (mentor), Mehle, Nataša (comentor)

'Candidatus Phytoplasma solani' je povzročiteljica bolezni na nekaterih gospodarsko pomembnih kulturnih rastlinah, med njimi tudi na vinski trti. Za namen proučevanja epidemiologije smo v okviru magistrske naloge molekularno opredelili vzorce DNA, izolirane iz okuženih rastlin Primorske in Podravske vinorodne dežele. V nalogi smo analizirali 124 vzorcev DNA, izoliranih iz okuženih rastlin (117 vzorcev vinske trte, 5 vzorcev koprive in 2 vzorca korenja), vzorčenih v obdobju med leti 2003 in 2016. Z analizo RFLP produktov ugnezdenih reakcij PCR ohranjenega gena tuf, smo vzorce razdelili v dve skupini: tuf-a in tuf-b. Vzorce iz skupine tuf-b smo na podlagi ene razlike v nukleotidnem zaporedju nadalje razdelili na podtipa tuf-b1 in tuf-b2. Ugotovili smo, da sta tuf-a in tuf-b prisotna na vseh preiskovanih vinorodnih območjih, pri čemer je tuf-b pogostejši, prevladuje tako v Primorski (52 %) kot Podravski (81 %) vinorodni deželi. Delež tuf-a je v Primorski vinorodni deželi višji (48 %) v primerjavi s Podravsko (19 %). Podtip tuf-b1 smo določili pri 62 %, tuf-b2 pa pri 38 % od vseh 71 analiziranih vzorcev tipa tuf-b. Podtip tuf-b2 je razen ene izjeme prisoten izključno v Podravski vinorodni deželi, prvič ga zaznamo leta 2007, z leti pa postaja vse pogostejši.

• Language: Slovenian
• Keywords: fitoplazme, Candidatus Phytoplasma solani, bolezen trsnih rumenic, vinska trta, vinorodne dežele, Slovenija, molekularne tehnike, PCR, RFLP, tuf
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Publisher: [S. Vidmar]
• Year: 2017
• PID: 20.500.12556/RUL-96391
• UDC: 632.35:634.8:577.2.083.1
• COBISS.SI-ID: 4821112
• Publication date in RUL: 30.09.2017

Secondary language

• Language: English
• Title: Molecular diversity of populations phytoplasma 'Candidatus Phytoplasma solani' in Slovene grapevine regions

Abstract

'Candidatus Phytoplasma solani' is responsible for various different diseases on important crop plants including grapevine, where it causes bois noir disease. For the epidemiologic purposes we preformed a molecular characterisation of DNA samples from infected plants from two grapevine regions in Slovenia: Primorje (south-west) and Podravje (north-east). We analysed 124 different DNA samples from infected plants, 117 of them were from vinegrape, 5 from stinging nettle and 2 samples from carrot. Samples were taken in time period between 2003 and 2016. With RFLP analysis of the nested PCR product of conserved gene tuf, we divided the samples into two groups: tuf-a and tuf-b. Samples from the tuf-b group have been further divided into tuf-b1 and tuf-b2 subgroup (based on a difference in one nucleotide in a gene sequence). We have determined that both tuf-a and tuf-b are present in all investigated regions, with tuf-b being more abundant in both regions: 52 % in SW and 81 % in NE, but tuf-a being more present in SW (48 %) than NE (19 %). Subtype tuf-b1 was found in 62 % and tuf-b2 in 38 % out of 71 analysed tuf-b samples. Subtype tuf-b2 is with only one exeption present exclusively in NE region. Tuf-b2 is first detected in NE region in 2007 and is becoming more abundant each year.

• Keywords: phytoplasmas, 'Candidatus Phytoplasma solani', bois noir, grapevine, grapevine regions, Slovenia, molecular techniques, PCR, RFLP, tuf