Vpliv slanosti na tvorbo biomase vrb v evapotranspiracijskih sistemih : poročilo o raziskavi

Podvršnik, Sandra (author), Istenič, Darja (mentor), Griessler Bulc, Tjaša (co-mentor)

Uvod: Evapotranspiracijski sistemi (ETS) sodijo med čistilne naprave za obdelavo in odstranjevanje komunalnih odpadnih voda, kjer se vsa odpadna voda porabi za rast rastlin, kot so vrbe. V ETS se sčasoma povečajo koncentracije soli (NaCl), kar vpliva na učinkovitost obdelave odpadne vode in rast vrb. Namen: S pomočjo lončnega poskusa smo želeli ugotoviti vpliv različnih koncentracij NaCl na rast in evapotranspiracijo vrb v ETS in primerjati odzive na slanost 3 različnih klonov vrb. Metode dela: Lončni poskus je potekal v rastlinjaku na 3 klonih bele vrbe (Salix alba) v 5 paralelkah in kontrolno serijo brez NaCl (skupaj 90 vzorcev). Testirali smo naslednje koncentracije NaCl: 0,1 g/L; 0,2 g/L; 0,5 g/L in 1,0 g/L. Beležili smo dnevno porabo vode in podatke statistično obdelali z računalniškim programom Microsoft Excel 2010, kjer smo razlike ugotavljali z analizo variance (ANOVA). Z računalniškim programom SPSS Statistics 17.0 smo za ugotavljanje značilnosti razlik uporabili t-test in za analizo povezanosti Pearsonov korelacijski koeficient. Iz absolutne porabe vode smo izračunali stopnjo normalizirane relativne transpiracije (NRT). Na koncu poskusa smo izmerili višino, debelino poganjkov, prešteli število poganjkov in naredili grafično primerjavo med njimi. Rezultati: Klon V160 je pri vseh koncentracijah NaCl porabil največ vode. Pri klonu V093 je bila največja NRT pri koncentraciji NaCl 1,0 g/L, pri klonu V160 pri koncentraciji 0,2 in 0,5 g/L in pri klonu V052 pri koncentraciji 0,5 g/L. Pri klonu V052 je bila NRT pri koncentraciji NaCl 0,2 g/L od začetka poskusa nižja od 100 %. Razprava in sklep: V našem poskusu se NaCl ni nakopičil v zemljo do te mere, da bi zaviral transpiracijo in vplival na rast vrb. Na podlagi pridobljenih rezultatov lahko pričakujemo, da pri klonih vrbe S. alba V093, V160 in V052 ne bi prišlo do vplivov NaCl na učinkovitost obdelave odpadne komunalne vode v realnem ETS pri koncentraciji NaCl do 1,0 g/L v 1 vegetacijski dobi. Predvidevamo, da bi se lahko rezultati v realnih ETS razlikovali od naših, saj nismo uporabili odpadne komunalne vode, ki bi lahko povečala privzem vode in pospešila kopičenje NaCl. Poleg tega bi bilo smiselno spremljati odziv vrb in medsebojno delovanje različnih dejavnikov na rast vrb več vegetacijskih dob.

• Language: Slovenian
• Keywords: vpliv slanosti, vrbe, Salix alba, evapotranspiracijski sistem, biomasa
• Work type: Master's thesis/paper
• Organization: ZF - Faculty of Health Sciences
• Year: 2017
• PID: 20.500.12556/RUL-95291
• COBISS.SI-ID: 5344363
• Publication date in RUL: 20.09.2017

Secondary language

• Language: English
• Title: The impact of salinity on the production/formation of willow biomass in the evapotranspiration systems : research report

Abstract

Introduction: Evapotranspiration systems (ETS) are among the cleaning plants for treatment and disposal of municipal wastewater, where all wastewater is used to grow plants such as willows. In the ETS, the concentration of salt (NaCl) eventually increases, which has an impact on the efficiency of wastewater treatment and the growth of willow trees. Purpose: With the help of pot experiment, we wanted to determine the impact of different concentrations of NaCl on growth and evapotranspiration of willows in the ETS and compare responses of salinity of 3 different clones of willow trees. Working methods: The pot experiment took place in the greenhouse on 3 clones of White Willow (Salix alba) in the 5 test portions and control batch without NaCl (a total of 90 samples). We tested the following concentrations of NaCl: 0.1 g/L; 0.2 g/L; 0.5 g/L and 1.0 g/L. We took notes of daily water usage and statistically processed the data with a computer, using Microsoft Excel 2010, where we tried to figure out the differences by analysis of variance (ANOVA). With the computer program SPSS 17.0, we used t-test for determination of difference characteristics and Pearson's correlation coefficient for analysis of connectedness. We calculated the level of normalized relative transpiration (the NRT) from absolute water consumption. At the end of the experiment, we measured height, thickness of the shoots and counted the number of shoots and made a graphic comparison between them. The results: The clone V160 used most of water with all concentrations of NaCl. In clone V093, NRT was the highest with the NaCl concentration of 1.0 g/L, in clone V160 at concentration 0,2 and 0.5 g/L, and in clone V052 at concentration 0.5 g/L. In clone V052, at NaCl concentration of 0.2 g/L, NRT was less than 100% from the beginning of the experiment. Discussion and conclusion: In our experiment, NaCl did not pile up in the ground to such extent that it would slow down the transpiration and affect the growth of willow trees. Based on the obtained results, we can expect that in S. alba clones V093, V160 and V052 it would not come to effects of NaCl on the efficiency of treatment of urban wastewater in real ETS at a concentration of NaCl up to 1.0 g/L in 1 vegetative period. We assume that the results in real ETS could differ from ours, because we did not use wastewater that could boost the uptake of water and speed up the accumulation of NaCl. In addition, it would make sense to monitor the response of willow trees and the interaction of various factors on the growth of willow trees during more vegetative periods.

• Keywords: influence of salinity, willow, Salix alba, evapotranspiration system, biomass