Introduction: The rapid development of the field of orthotics and prosthetics and the use of new materials and manufacturing processes can lead to adverse short and long term consequences on the health. of workers in orthotics and prosthetics workshops. Purpose: The aim of the study was to measure the physical and chemical parameters of work environment and determine their values in order to propose necessary measures intended for the potential improvement of work environment. Methods: In two selected orthotics and prosthetics workshops measurements of microclimate, noise, illumination, dust concentration and carbon dioxide concentration were carried out. The measuring points were determined in advance and measurements with five different measuring instruments were recorded. The obtained values were then compared to the set limit values and the compliance with the formal requirements was checked. Results: The results show that in both workshops most of the obtained microclimate values are in accordance with the formal requirements. The temperature in the private O&P centre, however, was 17 ºC, which is unsuitable. Also, exposure to noise exceeded the limit of 87 dB in both workshops during the use of a saw blade and a compressor. The concentration of dust particles was lower than the limit value of 6 mg/m3 at most measuring points. Higher concentration was recorded at the measuring point where polyurethane and plaster were sanded and cut. The CO2 concentration at Faculty of Health Sciences laboratory was 680 ppm and the CO2 concentration between 502 ppm and 1708 ppm was detected at the private O&P center, which is still considerably lower than the 5000 ppm limit. Based on the PMV and PPD results, more favourable thermal conditions prevailed in the private center of O&P. At the Faculty of Health Sciences laboratory, the PMV required limit was exceeded at the half of the measuring points. In the private O&P centre, the PMV was above the limit in the section D and ambulatory care area. Discussion and conclusion: The data collected from the current study suggest that during the use of machine tools, exposure to high levels of occupational noise can reduce hearing acuity and may be detrimental to the health of orthotists and prosthetists. To acquire the actual daily noise exposure repeated measurements with personal dosimeters would be required. Working conditions were similar and seemed appropriate in both workshops. Based on the findings of this study, we recommend the use of the personal protective equipment for hearing and respiratory protection.